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|Tested species reactivity||Yeast|
|Published species reactivity||Yeast|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||0.5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Syntaxins belong to a family of SNARE proteins. The yeast Saccharomyces cerevisiae has about 7 genes similar to syntaxin and there are presently 15 types of syntaxin genes in the human genome. In presence of other cytoplasmic proteins like the N-ethylmaleimide-sensitive factor (NSF) and SNAP; synatxins can combine various vesicular transport systems for the exocytic and endocytic pathway. This is very important for the functioning and for bringing about specificity to the vesicular combination process.. The syntaxin protein in yeast is also known as the Pep12 protein. The function of this Pep12 protein in the yeast is to transport vesicles from the Golgi apparatus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Proteomic profiling of autophagosome cargo in Saccharomyces cerevisiae.
A-21273 was used in western blot to describe a method for monitoring intact autophagosomes ex vivo.
|Suzuki K,Nakamura S,Morimoto M,Fujii K,Noda NN,Inagaki F,Ohsumi Y||PloS one (9:null)||2014|
Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy.
A-21273 was used in western blot to investigate the effects of sertraline/Zoloft® on the cell physiology of Saccharomyces cerevisiae.
|Chen J,Korostyshevsky D,Lee S,Perlstein EO||PloS one (7:null)||2012|
YOR036W, VPT13, VPS6, VPL6
Pep12p, VPS6, VPT13