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TAP Antibody (PA5-76897) in ICC/IF
Immunofluorescence analysis of Nuclear RNA export factor 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TAP Polyclonal Antibody (Product # PA5-76897) at 1:50 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product ... View More
Product Details
PA5-76897
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human TAP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2720624
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
The constitutive transport element (CTE) of type D retroviruses serves as a signal of nuclear export for unspliced viral RNAs. TAP (also known as NXF1) mediates the export of CTE-containing simian type D retroviral RNAs through binding directly to the CTE. TAP is associated with a recognized mRNA export pathway and is a member of the multigene family of NXF proteins. NXF proteins belong to an evolutionarily conserved family of proteins, which are characterized by a leucine-rich-repeat domain (LRR) followed by a region known as the Nuclear Transport Factor 2 (NTF2)-like domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mex 67 homolog; modifier of vibrator 1; mRNA export factor TAP; Nuclear RNA export factor 1; nuclear RNA export factor 1 homolog; tip associated protein; tip associating protein; Tip-associated protein; Tip-associating protein; Vdp
Gene Aliases: MEX67; Mex67h; Mvb1; NXF1; TAP
UniProt ID: (Human) Q9UBU9, (Rat) O88984, (Mouse) Q99JX7
Entrez Gene ID: (Human) 10482, (Rat) 59087, (Mouse) 53319
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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