Proteintech

TARS Polyclonal Antibody

Product Image Gallery

TARS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

TARS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14773-1-AP ( TARS Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TARS Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

TARS Antibody in Immunoprecipitation (IP) — IP result of anti-TARS (IP:14773-1-AP, 4ug; Detection:14773-1-AP 1:500) with A549 cells lysate 2400ug.

Product Details

14773-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TARS Fusion Protein Ag6614 (394-723 aa encoded by BC000517) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: ENMFSFEVE KELFALKPMN CPGHCLMFDH RPRSWRELPL RLADFGVLHR NELSGALTGL TRVRRFQQDD AHIFCAMEQI EDEIKGCLDF LRTVYSVFGF SFKLNLSTRP EKFLGDIEVW DQAEKQLENS LNEFGEKWEL NSGDGAFYGP KIDIQIKDAI GRYHQCATIQ LDFQLPIRFN LTYVSHDGDD KKRPVIVHRA ILGSVERMIA ILTENYGGKW PFWLSPRQVM VVPVGPTCDE YAQKVRQQFH DAKFMADIDL DPGCTLNKKI RNAQLAQYNF ILVVGEKEKI SGTVNIRTRD NKVHGERTIS ETIERLQQLK EFRSKQAEEE F (394-723 aa encoded by BC000517)

Target Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Threonyl-tRNA synthetase belongs to the class-II aminoacyl-tRNA synthetase family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC9344; threonine tRNA ligase 1, cytoplasmic; Threonine--tRNA ligase 1, cytoplasmic; Threonine--tRNA ligase, cytoplasmic; Threonyl-tRNA synthetase; Threonyl-tRNA synthetase 1; threonyl-tRNA synthetase, cytoplasmic; ThrRS

Gene Aliases: D15Wsu59e; TARS; TARS1; ThrRS

UniProt ID: (Human) P26639, (Mouse) Q9D0R2, (Rat) Q5XHY5

Entrez Gene ID: (Human) 6897, (Mouse) 110960, (Rat) 294810

Molecular Function: aminoacyl-tRNA synthetase