|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||a 22 amino acid peptide from near the carboxy terminus of human TBC1D1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-4 µg/ml|
A suggested positive control is Daudi cell lysate.
PA5-20389 can be used with blocking peptide PEP-0506.
TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain and presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2/USP6, yeast Bub2, and CDC16. TBC1D1 and TBC1D4 (AS160) have been demonstrated to be Rab GAPs (GTPase-activating proteins) that link upstream to Akt and phosphoinositide 3-kinase and downstream to Rabs involved in trafficking of GLUT4 vesicles. TBC1D1 regulates insulin-mediated GLUT4 translocation through its GAP activity, and is a likely Akt substrate. Mutations in the Tbc1d1 gene lead to some cases of severe human obesity.
TBC, TBC1; TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
KIAA1108; TBC; TBC1; TBC1D1