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Immunohistochemistry analysis of TBX3 in mouse E16.5 lung sections. Antigen retrieval from 4% PFA, paraffin embedded sections was performed using EDTA. Following antigen retrieval, tissues were blocked and probed with a TBX3 polyclonal antibody (Product # 42-4800) at a dilution of 1:1000. Detection was performed using an Alexa Fluor-568-conjugated anti-rabbit secondary antibody (A11036). Data courtesy of Dr. James Bridges at Cincinnati Children's Hospital Medical Center.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of human T-box 3, which differs from mouse and rat by one conservative and one non-conservative amino acid|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-2µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
42-4800 has been successfully used in immunohistochemistry analysis of Tbx3 in mouse E16.5 frozen lung sections.
T-box 3 (TBX3) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. The Tbx3 protein is a transcriptional repressor that binds the canonical Brachychury bindig sites, and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Tbx3 is required for normal mammary development and is also implicated in tumor development. Tbx3 expression also increases during osteoblast differentiation, and may function as a determinant of osteoblast cell numbers. Mutations in T-box 3 cause ulnar-mammary syndrome (MIM 181450), affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing results in three transcript variants encoding different isoforms; however, the nature of one full length variant has not been determined. Subsets of human breast cancer cell lines overexpress TBX36. in vitro, Tbx3 overexpression in mouse embryo fibroblasts leads to immortalization of cells. Truncated forms of Tbx3 are increased in plasma samples.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The anti-proliferative function of the TGF-β1 signaling pathway involves the repression of the oncogenic TBX2 by its homologue TBX3.
42-4800 was used in western blot to investigate the role of TBX2 and TBX3 on the anti-proliferative and pro-migratory roles of TGF-beta1
|Li J,Ballim D,Rodriguez M,Cui R,Goding CR,Teng H,Prince S||The Journal of biological chemistry (289:35633)||2014|
The ulnar-mammary syndrome gene, Tbx3, is a direct target of the retinoic acid signaling pathway, which regulates its expression during mouse limb development.
42-4800 was used in western blot to investigate how retinoic acid activates endogenous TBX3 expression.
|Ballim RD,Mendelsohn C,Papaioannou VE,Prince S||Molecular biology of the cell (23:2362)||2012|
Basal transcription of the human TBX3 gene, a key developmental regulator which is overexpressed in several cancers, requires functional NF-Y and Sp1 sites.
42-4800 was used in western blot to elucidate the mechanisms regulating TBX3 expression.
|Smith J,Mowla S,Prince S||Gene (486:41)||2011|
Transcriptional repressor Tbx3 is required for the hormone-sensing cell lineage in mammary epithelium.
42-4800 was used in immunohistochemistry - paraffin section to determine the role of Tbx3 in different cell types present in the adult mammary gland.
|Kunasegaran K,Ho V,Chang TH,De Silva D,Bakker ML,Christoffels VM,Pietersen AM||PloS one (9:null)||2014|
bladder cancer related protein XHL; T-box 3 (ulnar mammary syndrome); T-box protein 3; T-box transcription factor TBX3
TBX3; TBX3-ISO; UMS; XHL