Invitrogen

TCF3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (17) TCF3 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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TCF3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of TCF3 in human testis using a TCF3 polyclonal antibody (Product # PA5-84553). The analysis shows distinct nuclear positivity in cells in seminiferous ducts.

TCF3 Antibody in Western Blot (WB) — Western blot analysis of TCF3 by a TCF3 polyclonal antibody (Product # PA5-84553). Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line Daudi.

TCF3 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF-7 were transfected with, control siRNA, TCF3 siRNA (probe #1), and TCF3 siRNA (probe #2) and a decrease in signal intensity was observed in Western Blot application using anti-TCF3 Polyclonal Antibody (Product # PA5-84553) used at a dilution of 0.4 µg/ml. Anti-GAPDH was used as a loading control. {KD}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human TCF3

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2791704

Product Specific Information

Immunogen sequence: LAGSTSLMHN HAALPSQPGT LPDLSRPPDS YSGLGRAGA

Target Information

TCF3 (E2A) is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHb21; Class B basic helix-loop-helix protein 21; helix-loop-helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; MGC129647; MGC129648; negative vitamin D response element-binding protein; NOL1-TCF3 fusion; TCF-3; Transcription factor 3; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); transcription factor 3 variant 3; Transcription factor E2-alpha; Transcription factor ITF-1; VDR interacting repressor; vitamin D receptor-interacting repressor

Gene Aliases: AGM8; BHLHB21; E2A; E47; ITF1; TCF-3; TCF3; VDIR

UniProt ID: (Human) P15923

Entrez Gene ID: (Human) 6929

Molecular Function: basic helix-loop-helix transcription factor nucleic acid binding transcription factor