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Invitrogen
TCP1 Monoclonal Antibody (91A)
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FIGURE: 1 / 6
TCP1 Antibody (MA3-026) in ICC/IF
Immunofluorescent analysis of TCP1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TCP1 Monclonal Antibody (91A) (Product # MA3-026) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a f... View More
Product Details
MA3-026
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Hamster,
Human,
Mouse,
Non-human primate,
Rat,
Yeast
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
91A
Immunogen
Bacterially expressed fragment of mouse TCP-1, residues 306-556.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-026 detects T Complex Polypeptide 1 (TCP-1) from human, canine, chicken, hamster, mouse, primate, rat and yeast samples.
MA3-026 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this clone detects a ~57 kDa band representing TCP-1 in mouse 3T3 cells. Immunofluorescence staining of TCP-1 in mouse germ line cells with MA3-026 results in diffuse cytoplasmic staining.
The MA3-026 immunizing peptide corresponds to amino acid residues 306-556 from mouse TCP-1. The epitope for this antibody has been mapped to amino acids 465-469 representing the pentamer peptide AKLRA.
Target Information
The T Complex Polypeptide 1 (TCP-1) is approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 65 kDa antigen; CCT-alpha; Chaperonin containing T-complex polypeptide 1 subunit 1; t-complex 1 protein; t-complex polypeptide 1; T-complex protein 1 subunit alpha; T-complex protein 1 subunit alpha A; T-complex protein 1 subunit alpha B; T-complex protein 1, alpha subunit; tailless complex polypeptide 1; Tailless complex polypeptide 1A; Tailless complex polypeptide 1B; TCP-1-A; TCP-1-alpha; TCP-1-B
Gene Aliases: AI528772; c-cpn; CCT; CCT-alpha; CCT1; CCTA; CCTalpha; D6S230E; IDDPMGS; p63; Tcp-1; TCP-1-alpha; TCP1; Tp63; TRic; YD8142.13; YD8142B.04; YDR212W
protein folding
tubulin complex assembly
binding of sperm to zona pellucida
positive regulation of telomere maintenance via telomerase
protein stabilization
scaRNA localization to Cajal body
positive regulation of establishment of protein localization to telomere
positive regulation of protein localization to Cajal body
positive regulation of telomerase RNA localization to Cajal body
spermatid development
translocation of peptides or proteins into host cell cytoplasm
cellular protein metabolic process
toxin transport
regulation of macrophage apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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