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TCTEX1D2 Antibody (PA5-116015) in ICC/IF
Immunocytochemistry analysis of TCTEX1D2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116015) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TCTEX1D2(Accession Q8WW35), corresponding to amino acid residues E99-Y142.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2900649
Product Specific Information
Antibody detects endogenous levels of total TCTEX1D2.
Target Information
Dyneins are multi-subunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein is an approximately 12 subunit complex of two heavy chains, two intermediate chains to anchor Dynein to its cargo, four smaller intermediate chains and several light chains. It performs functions necessary for cell survival such as organelle transport and centrosome assembly. TCTEX1 is a cytoplasmic dynein light chain found in a complex with Na+ CP type X (SCN10A). TCTEX1D2, also called TCTEX1 domain containing 2, belongs to the dynein light chain TCTEX-type family. Its function is still under investigation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dynein light chain Tctex-type protein 2B; Tctex1 domain-containing protein 2
Gene Aliases: 0610012D17Rik; 2410011K15Rik; DYNLT2B; RGD1563597; TCTEX1D2
UniProt ID: (Human) Q8WW35, (Mouse) Q9CQ66
Entrez Gene ID: (Human) 255758, (Mouse) 66061, (Rat) 498095
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