Immunohistochemistry analysis of TEF1 in paraffin-embedded human placenta. Samples were incubated with TEF1 polyclonal antibody (Product # PA5-37075) using a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

TEF1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

TEF1 Antibody — Fig. 3 CRISPR-Cas9 ablation of TEAD1/4 inhibits migration in primary GBM cells. a Western immunoblot confirms population knockout of TEAD1 and TEAD4 after CRISPR-Cas9-mediated gene ablation. b Cell growth analysis reveals significantly decreased proliferation in TEAD1KO cells at 48-72 h, compared to sham (n = 3; 48 h: **p = 0.008; 72 h: *p = 0.01. Bars represent mean ± SEM). c Neurosphere (NS) assays show no difference in sphere number (day 6; n = 3 wells, multiple NS per well. Bars represent mean ± SEM). d Neurosphere (NS) assays show decreased sphere size in TEAD1 knockout, compared to sham. (day6; n = 3 wells, multiple NS per well; TEAD1KO: **p = 0.002. Dots represent individual NS and lines delineate mean). e Transwell invasion assays show decreased percent cell invasion in TEAD1KO and TEAD4KO cells, compared to sham (24 h; n = 3 wells; TEAD1KO: **p = 0.002; TEAD4KO: **p = 0.001. Bars represent mean ± SEM). On right, representative images of transwell invasion chamber membranes are shown. f, g Spheroid migration assays show decreased area of confluent cell migration (dispersion) at 36 h in TEAD1KO and TEAD4KO cells, compared to sham (f, PDL substrate), with partial rescue of migratory deficits in TEAD1KO cells after TEAD1 overexpression (OE) (g, laminin + PDL substrate) (n = 3 wells, with multiple NS per well; TEAD1KO: ***p = 0.0001; TEAD4KO: ***p = 0.00007; TEAD1OE 1: ***p = 0.00058; TEAD1OE 2 (1/10 dilution of TEAD1OE 1): ***p = 0.00015. Bars represent mean ± SEM). On r {KO}

Immunogen image

Immunogen Image TEF1 Polyclonal Antibody

Product Details

PA5-37075

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

ChIP assay (ChIP)

1:20-1:50

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

C. elegans

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 105-150 of human TEF-1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2553899

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 48 kDa.

Purity is >95% by SDS-PAGE.

Target Information

TEF1 (Transcriptional enhancer factor 1), a member of the TEA/ATTS domain family, is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes. TEF family members have a highly conserved DNA-binding domain TEF-1 binds to GT-IIC, SphI/II and M-CAT. TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer. Furthermore, TEF-1 represses transcription in placental cells. In vitro, TEF-1 is phosphorylated by several PKC isozymes. TEF-1 is phosphorylated in vivo at serine and threonine residues. Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition. TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis. Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: N TEAD1; NTEF-1; NTEF1; Protein GT-IIC; TCF-13; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEAD-1; Transcription factor 13; transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1

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Gene Aliases: 2610024B07Rik; AA; B230114H05Rik; Gtrgeo5; mTEF-1; NTEF-1; REF1; TCF-13; TCF13; TEAD-1; TEAD1; TEF-1; TEF1

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UniProt ID: (Human) P28347

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Entrez Gene ID: (Human) 7003, (Mouse) 21676, (Rat) 361630

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Function(s)

transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter proximal region DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA-binding transcription factor

Process(es)

transcription initiation from RNA polymerase II promoter hippo signaling positive regulation of pri-miRNA transcription from RNA polymerase II promoter embryonic heart tube morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter heart development transition between fast and slow fiber notochord development regulation of cell proliferation positive regulation of transcription from RNA polymerase II promoter paraxial mesoderm development lateral mesoderm development cellular response to retinoic acid positive regulation of protein serine/threonine kinase activity positive regulation of stem cell population maintenance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TEF1 Polyclonal Antibody

TEF1 Antibody (PA5-37075) in IHC (P)

Product Details

PA5-37075

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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