Invitrogen

TET1 Polyclonal Antibody

Product Image Gallery

TET1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of TET1 in paraffin-embedded human normal prostate tissue using TET1 polyclonal antibody (Product # PA5-85489) at a dilution of 1:1000.

TET1 Antibody in Western Blot (WB) — Western blot analysis of TET1 in A) 293T whole cell lysate, B) whole cell lysate of DDDDK-human TET1-transfected 293T cells using TET1 polyclonal antibody (Product # PA5-85489) using 30 µg of sample at a dilution of 1:5000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

Assay-dependent

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

Assay-dependent

Western Blot (WB)

1:500-1:20,000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1.

Conjugate

Unconjugated

Form

Liquid

Concentration

3.66 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Keep as concentrated solution.

Positive Control: 293T, A431, HeLa, HepG2, 293T tagged-hTET1, DDDDK-human TET1-transfected 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

TET1 (tet oncogene 1), also known as LCX or CXXC6, is a 2,136 amino acid protein that localizes to the nucleus and contains one CXXC-type zinc finger. Expressed in adult ovary, thymus and skeletal muscle and also present in fetal lung, heart and brain, TET1 is thought to play a role in the development of fetal organs and may also be involvement in the pathoegenesis and metastasis of acute myeloid leukemia (AML). The gene encoding TET1 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman's syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bA119F7.1 antibody; CXXC finger 6; CXXC finger 6 antibody; CXXC zinc finger 6; CXXC zinc finger 6 antibody; CXXC-type zinc finger protein 6; CXXC-type zinc finger protein 6 antibody; CXXC6 antibody; FLJ10839 antibody; FLJ41442 antibody; KIAA1676 antibody; LCX antibody; Leukemia-associated protein with a CXXC domain; leukemia-associated protein with a CXXC domain antibody; Methylcytosine dioxygenase TET1; methylcytosine dioxygenase TET1 antibody; Ten-eleven translocation 1 gene protein; ten-eleven translocation 1 gene protein antibody; Ten-eleven translocation 1 gene protein homolog; ten-eleven translocation-1; ten-eleven translocation-1 antibody; tet oncogene 1; tet oncogene 1 antibody; TET1 antibody; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456

Gene Aliases: 2510010B09Rik; AA517754; bA119F7.1; BB001228; CXXC6; D10Ertd17e; KIAA1676; LCX; mKIAA1676; MLL-TET1; TET1; TET1-MLL

UniProt ID: (Human) Q7Z6B6, (Mouse) Q3URK3

Entrez Gene ID: (Human) 80312, (Mouse) 52463

Function(s)