Invitrogen

TET1 Polyclonal Antibody

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TET1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of TET1 was performed in Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TET1 Polyclonal Antibody (Product # PA5-85489) diluted at 1:1000. Blue: Hoechst 33342 staining.

TET1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of TET1 in paraffin-embedded human normal prostate tissue using TET1 polyclonal antibody (Product # PA5-85489) at a dilution of 1:1000.

TET1 Antibody in Western Blot (WB) — Western blot analysis of TET1 in A) 293T whole cell lysate, B) whole cell lysate of DDDDK-human TET1-transfected 293T cells using TET1 polyclonal antibody (Product # PA5-85489) using 30 µg of sample at a dilution of 1:5000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (IHC)

Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1.

Conjugate

Unconjugated

Form

Liquid

Concentration

6.07 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2792629

Product Specific Information

Keep as concentrated solution.

Positive Control: 293T, A431, HeLa, HepG2, 293T tagged-hTET1, DDDDK-human TET1-transfected 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CXXC finger 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; RP11-119F7.1; Ten-eleven translocation 1 gene protein; ten-eleven translocation 1 gene protein homolog; ten-eleven translocation-1; tet oncogene 1; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456

Gene Aliases: 2510010B09Rik; AA517754; bA119F7.1; BB001228; CXXC6; D10Ertd17e; KIAA1676; LCX; mKIAA1676; MLL-TET1; TET1; TET1-MLL

UniProt ID: (Human) Q8NFU7

Entrez Gene ID: (Human) 80312, (Mouse) 52463

Function(s)