Invitrogen

TET1 Polyclonal Antibody

Product Image Gallery

TET1 Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of TET1 in SH-SY-5Y cells using a TET1 Polyclonal antibody (Product # PA5-86010)..

TET1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of TET1 of paraffin-embedded Mouse brain tissue using a TET1 Polyclonal antibody (Product #PA5-86010).

TET1 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of TET1 in SH-SY-5Y cells using a TET1 Polyclonal Antibody (Product # PA5-86010) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:50-1:100

Immunocytochemistry (ICC)

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (IHC)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide (KLH-conjugated) within Human TET1 820-900 aa.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.4, with 1% BSA, 40% Glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CXXC finger 6; CXXC zinc finger 6; CXXC-type zinc finger protein 6; Leukemia-associated protein with a CXXC domain; Methylcytosine dioxygenase TET1; Ten-eleven translocation 1 gene protein; Ten-eleven translocation 1 gene protein homolog; ten-eleven translocation-1; tet oncogene 1; TET1 splice variant VP_DE4; TET1 splice variant VP_DE456

Gene Aliases: 2510010B09Rik; AA517754; bA119F7.1; BB001228; CXXC6; D10Ertd17e; KIAA1676; LCX; mKIAA1676; MLL-TET1; TET1; TET1-MLL

UniProt ID: (Human) Q7Z6B6, (Mouse) Q3URK3

Entrez Gene ID: (Human) 80312, (Mouse) 52463, (Rat) 309902

Function(s)