Invitrogen

TGN46 Polyclonal Antibody

5 Published Figures

4 References

View all (11) TGN46 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

TGN46 Antibody — Published figure using TGN46 polyclonal antibody (Product # PA5-23068)

TGN46 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of TGN46 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TGN46 Polyclonal Antibody (Product # PA5-23068) at 5 µg/ml in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

TGN46 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Trans-Golgi Network 46 using a polyclonal antibody (Product # PA5-23068).

TGN46 Antibody in Western Blot (WB) — Western blot was performed using TGN46 Polyclonal Antibody (Product # PA5-23068) and a 39 kDa band corresponding to TGN46 was observed across cell lines and tissues tested along with an uncharacterized band (*) at ~80 kDa. Modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), T98G (Lane 2), Mouse muscle (Lane 3), Rat muscle (Lane 4), Mouse skin (Lane 5) and Rat skin (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (PA5-23068, 1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:500

Immunofluorescence (IF)

1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Western Blot (WB)

1:100-1:2000

Published Applications

Immunofluorescence (IF)

See 2 publications below

Western Blot (WB)

See 1 publication below

Miscellaneous PubMed (Misc)

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to an internal region of the human TGOLN2 protein (within residues 200-350).

Conjugate

Unconjugated

Form

Liquid

Concentration

0.66 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 30% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11156003

Product Specific Information

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Target Information

The TGOLN2 protein may be involved in regulating membrane traffic to and from the trans-Golgi network. There are several isoforms, which are produced by alternative splicing. The upregulation of TGOLN2 has been indicated in association with suicide cases. However, protein analysis has not verified that there is any direct correlation between suicide and TGOLN2 without alcohol influence. It is a highly glycosylated protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 48; 51kD isoforms); hTGN48; hTGN51; TGN38; TGN38 homolog; TGN38B; TGN46; TGN48; TGN51; Trans-Golgi network glycoprotein 46; Trans-Golgi network glycoprotein 48; Trans-Golgi network glycoprotein 51; Trans-Golgi network integral membrane protein 2; Trans-Golgi network integral membrane protein TGN38; trans-Golgi network protein (46; trans-Golgi network protein (46, 48, 51kD isoforms); trans-golgi network protein 1; Trans-Golgi network protein 2; trans-Golgi network protein TGN51; TTGN2

Gene Aliases: TGN38; TGN38B; TGN41; TGN46; TGN48; TGN51; Tgoln1; TGOLN2; Ttgn1; TTGN2

UniProt ID: (Human) O43493, (Rat) P19814, (Mouse) Q62314

Entrez Gene ID: (Human) 10618, (Rat) 192152, (Mouse) 22135

Molecular Function: membrane traffic protein