Invitrogen

TGN46 Polyclonal Antibody

Product Image Gallery

TGN46 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of TGN46 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TGN46 Polyclonal Antibody (Product # PA5-23068) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

TGN46 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Trans-Golgi Network 46 using a polyclonal antibody (Product # PA5-23068).

TGN46 Antibody in Western Blot (WB) — Western blot was performed using TGN46 Polyclonal Antibody (Product # PA5-23068) and a 39 kDa band corresponding to TGN46 was observed across cell lines and tissues tested along with an uncharacterized band (*) at ~80 kDa. Modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), T98G (Lane 2), Mouse muscle (Lane 3), Rat muscle (Lane 4), Mouse skin (Lane 5) and Rat skin (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (PA5-23068, 1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

PA5-23068

Applications	Tested Dilution	Publications
Western Blot (WB)	1:100-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:400	-
Immunocytochemistry (ICC/IF)	1:500	View 5 publications 5 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide made to an internal region of the human TGOLN2 protein (within residues 200-350). View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 30% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11156003

Product Specific Information

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Target Information

IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1. and ASPP2. are activators of p53.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PML nuclear body-residing proteins sequentially associate with HPV genome after infectious nuclear delivery.

PLoS pathogens

Guion L,Bienkowska-Haba M,DiGiuseppe S,Florin L,Sapp M

PA5-23068 was used in Immunocytochemistry-immunoflourescence to test the hypothesis that PML protein is recruited to incoming viral genome prior to egress from transport vesicles.

Fri Feb 01 00:00:00 EST 2019

The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.

Developmental cell

Procter DJ,Banerjee A,Nukui M,Kruse K,Gaponenko V,Murphy EA,Komarova Y,Walsh D

PA5-23068 was used in Immunocytochemistry to show that the assembly compartment also acts as a microtubule-organizing center (MTOC) wherein centrosome activity is suppressed and Golgi-based microtubule (MT) nucleation is enhanced.

Mon Apr 09 00:00:00 EDT 2018

Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection.

Open biology

Pavelin J,McCormick D,Chiweshe S,Ramachandran S,Lin YT,Grey F

Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

Cellular v-ATPase is required for virion assembly compartment formation in human cytomegalovirus infection.

Open biology

Pavelin J,McCormick D,Chiweshe S,Ramachandran S,Lin YT,Grey F

Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Immunofluorescence

Wed Nov 01 00:00:00 EDT 2017

Human Papillomavirus Major Capsid Protein L1 Remains Associated with the Incoming Viral Genome throughout the Entry Process.

Journal of virology

DiGiuseppe S,Bienkowska-Haba M,Guion LGM,Keiffer TR,Sapp M

PA5-23068 was used in Immunocytochemistry to demonstrate that human papillomavirus conformationally intact L1 protein, likely still arranged as capsomeres, is associated with the viral genome throughout mitosis and transiently resides in the nucleus until after the viral DNA is released from the transport vesicle.

Tue Aug 15 00:00:00 EDT 2017

Incoming human papillomavirus type 16 genome resides in a vesicular compartment throughout mitosis.

Proceedings of the National Academy of Sciences of the United States of America

DiGiuseppe S,Luszczek W,Keiffer TR,Bienkowska-Haba M,Guion LG,Sapp MJ

Published figure using TGN46 polyclonal antibody (Product # PA5-23068) in Immunofluorescence

Tue May 31 00:00:00 EDT 2016

Topography of the Human Papillomavirus Minor Capsid Protein L2 during Vesicular Trafficking of Infectious Entry.

Journal of virology

DiGiuseppe S,Keiffer TR,Bienkowska-Haba M,Luszczek W,Guion LG,Müller M,Sapp M

PA5-23068 was used in immunocytochemistry to map the topography of the human papillomavirus L2 protein during infection

Thu Oct 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: hTGN48; hTGN51; TGN38 homolog; Trans-Golgi network glycoprotein 46; Trans-Golgi network glycoprotein 48; Trans-Golgi network glycoprotein 51; Trans-Golgi network integral membrane protein 2; trans-Golgi network protein (46, 48, 51kD isoforms); Trans-Golgi network protein 2; trans-Golgi network protein TGN51

Gene Aliases: TGN38; TGN46; TGN48; TGN51; TGOLN2; TTGN2

UniProt ID: (Human) F8WBK2

Entrez Gene ID: (Human) 10618

Molecular Function: membrane traffic protein