|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 264 and 330 of Human TGR5|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
PA5-27076 targets TGR5 in IHC (P) and WB applications and shows reactivity with Human samples.
PA5-27076 targets TGR5 in IHC applications and shows reactivity with Mouse adrenal gland samples.
The PA5-27076 immunogen is synthetic peptide corresponding to a region within amino acids 264 and 330 of Human TGR5.
This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Enteroendocrine cells are a potential source of serum autotaxin in men.
PA5-27076 was used in immunohistochemistry - paraffin section to discover a potential source of serum autotaxin in men by analyzing enteroendocrine cells
|Bolier R,Tolenaars D,Kremer AE,Saris J,Parés A,Verheij J,Bosma PJ,Beuers U,Oude Elferink RP||Biochimica et biophysica acta (1862:696)||2016|