Proteintech

TIMM44 Polyclonal Antibody

TIMM44 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

TIMM44 Antibody (13859-1-AP) in ICC/IF

Immunofluorescent analysis of A549 cells, using TIMM44 antibody 13859-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

TIMM44 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13859-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TIMM44 Fusion Protein Ag4834 (153-452 aa encoded by BC033628)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AKTAKQSAE SVSKGGEKLG RTAAFRALSQ GVESVKKEID DSVLGQTGPY RRPQRLRKRT EFAGDKFKEE KVFEPNEEAL GVVLHKDSKW YQQWKDFKEN NVVFNRFFEM KMKYDESDNA FIRASRALTD KVTDLLGGLF SKTEMSEVLT EILRVDPAFD KDRFLKQCEN DIIPNVLEAM ISGELDILKD WCYEATYSQL AHPIQQAKAL GLQFHSRILD IDNVDLAMGK MMEQGPVLII TFQAQLVMVV RNPKGEVVEG DPDKVLRMLY VWALCRDQDE LNPYAAWRLL DISASSTEQI L (153-452 aa encoded by BC033628)

Target Information

This gene encodes a peripheral membrane protein associated with the mitochondrial inner membrane translocase, which functions in the import of proteins across the mitochondrial inner membrane and into the mitochondrial matrix. The encoded protein mediates binding of mitochondrial heat shock protein 70 to the translocase of inner mitochondrial membrane 23 (TIM23) complex. Expression of this gene is upregulated in kidney in a mouse model of diabetes. A mutation in this gene is associated with familial oncocytic thyroid carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria.

Cell reports

Akabane S,Watanabe K,Kosako H,Yamashita SI,Nishino K,Kato M,Sekine S,Kanki T,Matsuda N,Endo T,Oka T

Tue May 30 00:00:00 UTC 2023

Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy.

Nature communications

Michaelis JB,Brunstein ME,Bozkurt S,Alves L,Wegner M,Kaulich M,Pohl C,Münch C

Fri Sep 02 00:00:00 UTC 2022

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

Cell metabolism

Morgenstern M,Peikert CD,Lübbert P,Suppanz I,Klemm C,Alka O,Steiert C,Naumenko N,Schendzielorz A,Melchionda L,Mühlhäuser WWD,Knapp B,Busch JD,Stiller SB,Dannenmaier S,Lindau C,Licheva M,Eickhorst C,Galbusera R,Zerbes RM,Ryan MT,Kraft C,Kozjak-Pavlovic V,Drepper F,Dennerlein S,Oeljeklaus S,Pfanner N,Wiedemann N,Warscheid B

Tue Dec 07 00:00:00 UTC 2021

ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.

Human molecular genetics

Lin BC,Phung TH,Higgins NR,Greenslade JE,Prado MA,Finley D,Karbowski M,Polster BM,Monteiro MJ

Thu Jun 17 00:00:00 UTC 2021

ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.

Human molecular genetics

Lin BC,Phung TH,Higgins NR,Greenslade JE,Prado MA,Finley D,Karbowski M,Polster BM,Monteiro MJ

Thu Jun 17 00:00:00 UTC 2021

ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.

Human molecular genetics

Lin BC,Phung TH,Higgins NR,Greenslade JE,Prado MA,Finley D,Karbowski M,Polster BM,Monteiro MJ

Thu Jun 17 00:00:00 UTC 2021

ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.

Human molecular genetics

Lin BC,Phung TH,Higgins NR,Greenslade JE,Prado MA,Finley D,Karbowski M,Polster BM,Monteiro MJ

Thu Jun 17 00:00:00 UTC 2021

LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.

Nature communications

Shin CS,Meng S,Garbis SD,Moradian A,Taylor RW,Sweredoski MJ,Lomenick B,Chan DC

Mon Jan 11 00:00:00 UTC 2021

Balancing of mitochondrial translation through METTL8-mediated m³C modification of mitochondrial tRNAs.

Molecular cell

Schöller E,Marks J,Marchand V,Bruckmann A,Powell CA,Reichold M,Mutti CD,Dettmer K,Feederle R,Hüttelmaier S,Helm M,Oefner P,Minczuk M,Motorin Y,Hafner M,Meister G

Thu Dec 02 00:00:00 UTC 2021

Super-resolution microscopy reveals the arrangement of inner membrane protein complexes in mammalian mitochondria.

Journal of cell science

Palmer CS,Lou J,Kouskousis B,Pandzic E,Anderson AJ,Kang Y,Hinde E,Stojanovski D

Thu Jul 01 00:00:00 UTC 2021

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.

eLife

Dennerlein S,Poerschke S,Oeljeklaus S,Wang C,Richter-Dennerlein R,Sattmann J,Bauermeister D,Hanitsch E,Stoldt S,Langer T,Jakobs S,Warscheid B,Rehling P

Fri Dec 31 00:00:00 UTC 2021

Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.

Molecular & cellular proteomics : MCP

Fielden LF,Scott NE,Palmer CS,Khoo CA,Newton HJ,Stojanovski D

Mon Mar 14 00:00:00 UTC 2022

Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.

Frontiers in cell and developmental biology

Mao X,Gu Y,Sui X,Shen L,Han J,Wang H,Xi Q,Zhuang Q,Meng Q,Wu G

Tue Apr 26 00:00:00 UTC 2022

Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.

eLife

Kang Y,Anderson AJ,Jackson TD,Palmer CS,De Souza DP,Fujihara KM,Stait T,Frazier AE,Clemons NJ,Tull D,Thorburn DR,McConville MJ,Ryan MT,Stroud DA,Stojanovski D

Mon Nov 04 00:00:00 UTC 2019

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Nucleic acids research

Lavdovskaia E,Kolander E,Steube E,Mai MM,Urlaub H,Richter-Dennerlein R

Wed Sep 19 00:00:00 UTC 2018

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Nucleic acids research

Lavdovskaia E,Kolander E,Steube E,Mai MM,Urlaub H,Richter-Dennerlein R

Wed Sep 19 00:00:00 UTC 2018

COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.

eLife

Aich A,Wang C,Chowdhury A,Ronsör C,Pacheu-Grau D,Richter-Dennerlein R,Dennerlein S,Rehling P

Tue Jan 30 00:00:00 UTC 2018

COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.

eLife

Aich A,Wang C,Chowdhury A,Ronsör C,Pacheu-Grau D,Richter-Dennerlein R,Dennerlein S,Rehling P

Tue Jan 30 00:00:00 UTC 2018

PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.

Nature cell biology

Saita S,Nolte H,Fiedler KU,Kashkar H,Venne AS,Zahedi RP,Krüger M,Langer T

Sat Apr 01 00:00:00 UTC 2017

PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.

Nature cell biology

Saita S,Nolte H,Fiedler KU,Kashkar H,Venne AS,Zahedi RP,Krüger M,Langer T

Sat Apr 01 00:00:00 UTC 2017

The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.

Development (Cambridge, England)

Rauschenberger V,Bernkopf DB,Krenn S,Jalal K,Heller J,Behrens J,Gentzel M,Schambony A

Thu Jun 15 00:00:00 UTC 2017

The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.

Development (Cambridge, England)

Rauschenberger V,Bernkopf DB,Krenn S,Jalal K,Heller J,Behrens J,Gentzel M,Schambony A

Thu Jun 15 00:00:00 UTC 2017

Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.

Cell

Richter-Dennerlein R,Oeljeklaus S,Lorenzi I,Ronsör C,Bareth B,Schendzielorz AB,Wang C,Warscheid B,Rehling P,Dennerlein S

Thu Oct 06 00:00:00 UTC 2016

Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.

Cell

Richter-Dennerlein R,Oeljeklaus S,Lorenzi I,Ronsör C,Bareth B,Schendzielorz AB,Wang C,Warscheid B,Rehling P,Dennerlein S

Thu Oct 06 00:00:00 UTC 2016

Translocase of inner mitochondrial membrane 44 alters the mitochondrial fusion and fission dynamics and protects from type 2 diabetes.

Metabolism: clinical and experimental

Wang Y,Katayama A,Terami T,Han X,Nunoue T,Zhang D,Teshigawara S,Eguchi J,Nakatsuka A,Murakami K,Ogawa D,Furuta Y,Makino H,Wada J

Mon Jun 01 00:00:00 UTC 2015

Translocase of inner mitochondrial membrane 44 alters the mitochondrial fusion and fission dynamics and protects from type 2 diabetes.

Metabolism: clinical and experimental

Wang Y,Katayama A,Terami T,Han X,Nunoue T,Zhang D,Teshigawara S,Eguchi J,Nakatsuka A,Murakami K,Ogawa D,Furuta Y,Makino H,Wada J

Mon Jun 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Mitochondrial import inner membrane translocase subunit TIM44; translocase of inner mitochondrial membrane 44 homolog; translocator of inner mitochondrial membrane 44; translocator of inner mitochondrial membrane 44; DNA segment, Chr 8, ERATO Doi 118, expressed

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Gene Aliases: 0710005E20Rik; D8Ertd118e; MIMT44; TIM44; TIMM44

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UniProt ID: (Human) O43615, (Mouse) O35857, (Rat) O35094

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Entrez Gene ID: (Human) 10469, (Mouse) 21856, (Rat) 29635

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Function(s)

protein binding ATP binding chaperone binding nucleotide binding P-P-bond-hydrolysis-driven protein transmembrane transporter activity enzyme binding transporter

Process(es)

protein targeting to mitochondrion protein import into mitochondrial matrix transport intracellular protein transport protein transport protein transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TIMM44 Polyclonal Antibody

TIMM44 Antibody (13859-1-AP) in ICC/IF

Product Details

13859-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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