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TIN2 Antibody (PA5-121036) in WB
Western blot analysis of TIN2 in extracts of various cell lines. Samples were incubated with TIN2 Polyclonal antibody (Product # PA5-121036) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-121036
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 202-451 of human TIN2/TIN2/TINF2 (NP_0010927441)
Conjugate
Form
Liquid
Concentration
1.61 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914608
Product Specific Information
Positive test controls include: MCF7, HepG2, Mouse skeletal muscle, Mouse heart. The target is usually found in the following locations: Chromosome, Nucleus, Nucleus matrix, telomere.
Immunogen sequence: ECSVTDSVNL AEPMEQNPPQ QQRLALHNPL PKAKPGTHLP QGPSSRTHPE PLAGRHFNLA PLGRRRVQSQ WASTRGGHKE RPTVMLFPFR NLGSPTQVIS KPESKEEHAI YTADLAMGTR AASTGKSKSP CQTLGGRALK ENPVDLPATE QKENCLDCYM DPLRLSLLPP RARKPVCPPS LCSSVITIGD LVLDSDEEEN GQGEGKESLE NYQKTKFDTL IPTLCEYLPP SGHGAIPVSS CDCRDSSRPL
Target Information
TRF1 is a negative regulator of telomere length maintenance. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous TIN2, like endogenousTRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: TERF1 (TRF1)-interacting nuclear factor 2; TERF1-interacting nuclear factor 2; tin; Tin-2; TRF1-interacting nuclear factor 2; TRF1-interacting nuclear protein 2
Gene Aliases: AW552114; D14Wsu146e; DKCA3; TIN2; TINF2
UniProt ID: (Human) Q9BSI4
Entrez Gene ID: (Human) 26277, (Mouse) 28113
telomere maintenance via telomere lengthening
negative regulation of protein ADP-ribosylation
telomere assembly
negative regulation of telomere maintenance
positive regulation of telomere maintenance
negative regulation of telomere maintenance via telomerase
protein localization to chromosome
negative regulation of epithelial cell proliferation
protein localization to chromosome, telomeric region
telomere capping
negative regulation of telomerase activity
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