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TMED5 Antibody (PA5-144635) in IHC (P)
Immunohistochemistry of TMED5 in paraffin-embedded mouse large intestine tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with TMED5 polyclonal antibody (Product # PA5-144635) with a dilution of 1:5,000 for 1 hour at roo... View More
Product Details
PA5-144635
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human TMED5 aa 1-200/229.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3094094
Product Specific Information
Positive control: Rat liver tissue lysate, A431 cell lysate, Jurkat cell lysate, Hela cell lysate, SiHa cell lysate, rat bladder tissue, mouse large intestine tissue.
Predicted band size: 26 kDa
Subcellular Location: Cis-Golgi network membrane, endoplasmic reticulum membrane, endoplasmic reticulum-Golgi intermediate compartment membrane.
Target Information
The TMED5 gene encodes transmembrane emp24 domain-containing protein 5, which is part of the p24 protein family involved in vesicular transport within the secretory pathway. TMED5 is predominantly localized in the endoplasmic reticulum and Golgi apparatus, where it plays a crucial role in the trafficking of secretory proteins. This protein is involved in the budding of transport vesicles and the selection of cargo molecules, assisting in their efficient transport through the secretory pathway. TMED5 is essential for maintaining cellular homeostasis and protein processing, impacting the secretion of various proteins that are vital for numerous physiological processes. Additionally, TMED5 has been implicated in pathological conditions related to protein misfolding and trafficking defects, highlighting its importance in cellular function and disease mechanisms. This gene's involvement in vesicular transport makes it a subject of interest, particularly in studies focusing on protein trafficking disorders and their therapeutic interventions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p24 family protein gamma-2; p24gamma2; p28; Transmembrane emp24 domain-containing protein 5; transmembrane emp24 protein transport domain containing 5
Gene Aliases: 3110020O18Rik; 4432412D15Rik; CGI-100; LRRG00120; p24g2; p28; TMED5; UNQ397/PRO733
UniProt ID: (Human) Q9Y3A6, (Rat) Q6AXN3, (Mouse) Q9CXE7
Entrez Gene ID: (Human) 50999, (Rat) 289883, (Mouse) 73130
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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