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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TMEM27 Antibodies

          Invitrogen

          TMEM27 Polyclonal Antibody

          View all (13) TMEM27 antibodies

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          Cite TMEM27 Polyclonal Antibody

          • Antibody Testing Data (3)
          TMEM27 Antibody in Immunohistochemistry (Frozen) (IHC (F))
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          TMEM27 Antibody in Immunohistochemistry (Frozen) (IHC (F))
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          FIGURE: 1 / 3

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          TMEM27 Antibody (PA5-47676) in IHC (F)

          Immunohistochemical analysis of TMEM27 in perfusion fixed frozen sections of mouse kidney. Samples were incubated with TMEM27 polyclonal antibody (Product # PA5-47676) using a dilution of 1.7 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubules. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          TMEM27 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          TMEM27 Antibody in Western Blot (WB)
          TMEM27 Antibody in Western Blot (WB)
          TMEM27 Polyclonal Antibody

          Product Details

          PA5-47676

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-10 µg/mL
          -

          Immunohistochemistry (Frozen) (IHC (F))

          5-15 µg/mL
          -
          Product Specifications

          Species Reactivity

          Mouse

          Host/Isotype

          Sheep / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Mouse myeloma cell line NS0-derived recombinant mouse Collectrin Glu15-Ile144
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          0.2 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 5% trehalose

          Contains

          No Preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2605557

          Product Specific Information

          In direct ELISAs, approximately 40% cross-reactivity with recombinant human Collectrin is observed.

          Reconstitute at 0.2 mg/mL in sterile PBS.

          Target Information

          TMEM27 (Transmembrane protein 27) is also named as collectrin and Belongs to the TMEM27 family. It is a stimulator of beta cell replication and a membrane protein cleaved and shed by pancreatic beta cells that has been proposed as a beta cell mass biomarker. Western blot analysis of mouse collecting duct cell lines detected Tmem27 at an apparent molecular mass of 32 kDa. TMEM27 is an N-glycosylated protein and forms dimers, with MW of 64-75 kDa.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Collectrin; kidney-specific membrane protein; Transmembrane protein 27

          View more View less

          Gene Aliases: 0610008J07Rik; Cltrn; NX-17; NX17; Tmem27

          View more View less

          UniProt ID: (Mouse) Q9ESG4

          View more View less

          Entrez Gene ID: (Mouse) 57394

          View more View less

          Function(s)
          protein binding metallopeptidase activity peptidyl-dipeptidase activity
          Process(es)
          proteolysis positive regulation of SNARE complex assembly positive regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of calcium ion-dependent exocytosis positive regulation of amino acid transport
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

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