|ELISA (ELISA)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Neutralization (Neu)||See 6 publications below|
|ELISA (ELISA)||See 5 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant mouse TNF alpha|
|Storage buffer||PBS with 4% BSA|
MM350DB targets TNF Alpha in ELISA, Neutralization and WB applications and shows reactivity with mouse samples.
The MM350DB immunogen is recombinant mouse TNF alpha. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
MM350DB detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
The MM350DB mouse TNFa antibody (biotinylated conjugate of clone XT22) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM350C (clone XT3). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody MM350DB (clone XT22) and antibody MM350C (clone XT3) have successfully been used in combination with recombinant mouse TNFa protein SMTNFA in ELISA applications.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cachectin; TNF alpha; TNF-a; TNF-alpha; Tumor necrosis factor; tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Gene Aliases: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2
UniProt ID: (Mouse) P06804
Entrez Gene ID: (Mouse) 21926
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