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          • Primary Antibodies ›
          • TNF alpha Antibodies

          Invitrogen

          TNF alpha Polyclonal Antibody

          Advanced Verification
          9 Published Figures
          25 References
          View all (226) TNF alpha antibodies

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          Cite TNF alpha Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (9)
          • Advanced Verification (3)
          TNF alpha Antibody in Immunocytochemistry (ICC/IF)
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          TNF alpha Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          TNF alpha Antibody (AMC3012) in ICC/IF

          Immunofluorescence analysis of TNF-Alpha (Tumor necrosis factor alpha) was performed using 70% confluent log phase RAW 264.7 treated with lipopolysaccharide (LPS) (10 ng/mL for 7 hr) and protein transport inhibitor cocktail [500X] (PTI) (1X for 4 hr). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 2% BSA for 45 minutes at r... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          TNF alpha Antibody in Immunocytochemistry (ICC/IF)
          TNF alpha Antibody in Western Blot (WB)
          TNF alpha Antibody in Immunocytochemistry (ICC/IF)
          TNF alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TNF alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TNF alpha Antibody in Immunohistochemistry (IHC)
          TNF alpha Antibody in Western Blot (WB)
          TNF alpha Antibody in Western Blot (WB)
          TNF alpha Antibody in Neutralization (Neu)
          TNF alpha Antibody in Neutralization (Neu)
          TNF alpha Antibody in Neutralization (Neu)
          TNF alpha Antibody
          TNF alpha Antibody
          TNF alpha Antibody

          Product Details

          AMC3012

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 7 publications 7 publications

          Immunohistochemistry (IHC)

          -
          View 8 publications 8 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          1:100
          View 2 publications 2 publications

          ELISA (ELISA)

          Assay-dependent
          View 2 publications 2 publications

          Neutralization (Neu)

          Assay-dependent
          View 3 publications 3 publications

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / Ig

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant mouse TNF-alpha produced in E. coli

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          1 mg/mL

          Amount

          500 µg

          Purification

          Protein A

          Storage buffer

          PBS with 125mM trehalose

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536391

          Product Specific Information

          Reconstitute using 0.5 mL sterile distilled water.

          Target Information

          TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: APC1 protein; Cachectin; cell apoptosis-related protein; DADB-70P7.1; TNF alpha; TNF superfamily; TNF superfamily member 2; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrotic factor alpha; unnamed protein product

          View more View less

          Gene Aliases: DIF; IMD127; TNF; TNF-a; TNF-alpha; TNFA; TNFalpha; Tnfsf1a; TNFSF2; TNLG1F

          View more View less

          UniProt ID: (Human) P01375, (Mouse) P06804

          View more View less

          Entrez Gene ID: (Human) 7124, (Mouse) 21926

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding protease binding cytokine activity tumor necrosis factor receptor binding protein binding death receptor agonist activity identical protein binding receptor agonist activity transcription regulatory region DNA binding
          Process(es)
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPKKK activity activation of MAPK activity cell activation positive regulation of cytokine production regulation of protein phosphorylation positive regulation of protein phosphorylation negative regulation of L-glutamate transport chronic inflammatory response to antigenic stimulus negative regulation of cytokine secretion involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin glucose metabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process obsolete transformed cell apoptotic process defense response inflammatory response immune response humoral immune response signal transduction JNK cascade multicellular organism development cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to virus organ morphogenesis positive regulation of gene expression negative regulation of gene expression negative regulation of alkaline phosphatase activity calcium-mediated signaling extracellular matrix organization osteoclast differentiation sequestering of triglyceride positive regulation of protein complex assembly positive regulation of fever generation lipopolysaccharide-mediated signaling pathway negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-18 production positive regulation of interleukin-6 production receptor biosynthetic process positive regulation of peptidyl-serine phosphorylation tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion regulation of cell proliferation positive regulation of NF-kappaB import into nucleus defense response to bacterium positive regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of protein complex disassembly positive regulation of protein complex disassembly positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity positive regulation of neuron apoptotic process negative regulation of growth of symbiont in host negative regulation of viral genome replication positive regulation of chemokine biosynthetic process cellular extravasation positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of myoblast differentiation negative regulation of osteoblast differentiation regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of action potential positive regulation of cell adhesion positive regulation of mitotic nuclear division positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import positive regulation of JNK cascade positive regulation of smooth muscle cell proliferation regulation of protein secretion positive regulation of cytokine secretion positive regulation of inflammatory response positive regulation of phagocytosis regulation of insulin secretion positive regulation of synaptic transmission defense response to Gram-positive bacterium leukocyte migration leukocyte tethering or rolling detection of mechanical stimulus involved in sensory perception of pain negative regulation of lipid catabolic process regulation of immunoglobulin secretion positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein transport response to glucocorticoid positive regulation of NFAT protein import into nucleus positive regulation of hair follicle development positive regulation of protein kinase B signaling positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of branching involved in lung morphogenesis cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to nicotine cellular response to organic cyclic compound positive regulation of podosome assembly apoptotic signaling pathway extrinsic apoptotic signaling pathway necroptotic signaling pathway positive regulation of protein localization to cell surface positive regulation of chemokine (C-X-C motif) ligand 2 production regulation of reactive oxygen species metabolic process negative regulation of extrinsic apoptotic signaling pathway in absence of ligand response to hypoxia microglial cell activation negative regulation of endothelial cell proliferation macrophage activation involved in immune response regulation of immunoglobulin production positive regulation of immunoglobulin production negative regulation of cytokine production involved in immune response skeletal muscle contraction negative regulation of systemic arterial blood pressure JAK-STAT cascade circadian rhythm response to xenobiotic stimulus response to mechanical stimulus response to bacterium response to salt stress response to fructose negative regulation of heart rate vascular endothelial growth factor production positive regulation of macrophage derived foam cell differentiation negative regulation of lipid storage response to activity regulation of metabolic process positive regulation of defense response negative regulation of myelination response to nutrient levels response to lipopolysaccharide positive regulation of fractalkine production positive regulation of interleukin-1 beta production positive regulation of interleukin-8 production toll-like receptor 3 signaling pathway positive regulation of cellular amide metabolic process response to isolation stress response to macrophage colony-stimulating factor NIK/NF-kappaB signaling vasodilation negative regulation of apoptotic process positive regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration innate immune response response to ethanol regulation of fat cell differentiation negative regulation of fat cell differentiation positive regulation of protein catabolic process positive regulation of lipid metabolic process negative regulation of mitotic cell cycle astrocyte activation embryonic digestive tract development regulation of inflammatory response negative regulation of neurogenesis regulation of developmental process modulation of synaptic transmission regulation of synapse organization cognition regulation of secretion regulation of multicellular organismal process regulation of synaptic transmission, glutamatergic positive regulation of glial cell proliferation negative regulation of vascular wound healing regulation of biological quality positive regulation of ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to molecule of bacterial origin cellular response to retinoic acid cellular response to interferon-gamma cellular response to ionizing radiation positive regulation of mononuclear cell migration endothelial cell apoptotic process protein localization to plasma membrane negative regulation of oxidative phosphorylation inflammatory response to wounding cellular response to toxic substance liver regeneration negative regulation of bile acid secretion antiviral innate immune response response to Gram-negative bacterium positive regulation of neuroinflammatory response positive regulation of interleukin-33 production positive regulation of cytokine production involved in inflammatory response negative regulation of beta-amyloid clearance positive regulation of NIK/NF-kappaB signaling positive regulation of synoviocyte proliferation positive regulation of beta-amyloid formation response to L-glutamate positive regulation of neutrophil activation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly positive regulation of cellular protein localization cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of leukocyte adhesion to arterial endothelial cell regulation of membrane lipid metabolic process response to 3,3',5-triiodo-L-thyronine response to gold nanoparticle positive regulation of blood microparticle formation positive regulation of hepatocyte proliferation regulation of endothelial cell apoptotic process positive regulation of DNA biosynthetic process negative regulation of apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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