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Invitrogen
Mouse TNF alpha Recombinant Protein
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Product Details
14-8321-62
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
5 µg
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Mouse TNF-a is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4^+ T cells and NK cells. A 26 kDa form of TNF-alpha is expressed as a membrane-bound molecule. TNF-alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-alpha have been observed.
Applications Reported: Recombinant mouse TNF alpha is biologically active and can induce cytolysis of the L929 mouse cell line in culture.
Applications Tested: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg.
Source: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF-alpha accession # NM_013693.
Bioactivity: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides on SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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