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TNNT3 Antibody (PA5-114785) in ICC/IF
Immunocytochemistry analysis of TNNT3 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114785). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-114785
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TNNT3(Accession P45378), corresponding to amino acid residues Q220-K269.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899421
Product Specific Information
Antibody detects endogenous levels of total TNNT3.
Target Information
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta TnTF; Beta-TnTF; Fast skeletal muscle troponin T; fast skeletal muscle troponin T3; fTnT; skeletal muscle fast-twitch TnT; TnTf; troponin T type 3 (skeletal, fast); Troponin T, fast skeletal muscle; troponin-T3, skeletal, fast
Gene Aliases: fTnT; TNNT3; TNTF
UniProt ID: (Human) P45378, (Mouse) Q9QZ47
Entrez Gene ID: (Human) 7140, (Mouse) 21957
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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