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Invitrogen
TOP3B Polyclonal Antibody
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FIGURE: 1 / 5
TOP3B Antibody (PA5-101080) in IHC (P)
Immunohistochemistry analysis of TOP3B in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with TOP3B polyclonal antibody (Product # PA5-101080) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-101080
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TOP3B(Accession O95985), corresponding to amino acid residues A817-V862.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TOP3B.
Target Information
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I and DNA topoisomerase II relax both positive and negative supercoils, DNA topoisomerase III relaxes only negative supercoils. DNA topoisomerase III alpha exists as a long and a short isoform, which are produced by alternative splicing. DNA topoisomerase III alpha, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. DNA topoisomerase III beta exists as three isoforms, namely beta1, beta2 and beta3, also produced by alternative splicing. DNA topoisomerase III beta1 is expressed in testes, heart and skeletal muscle, whereas beta2 is expressed in thymus, kidney and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1; Topo III beta; topoisomerase III beta
Gene Aliases: TOP3B; TOP3B1
UniProt ID: (Human) O95985, (Mouse) Q9Z321
Entrez Gene ID: (Human) 8940, (Mouse) 21976
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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