Invitrogen

TPR Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (16) TPR antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (7)
Advanced Verification (1)

TPR Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 8

TPR Antibody (PA5-54048) in IHC

Immunohistochemical staining of TPR in human colon, kidney, lymph node and testis using TPR Polyclonal Antibody (Product # PA5-54048) (A) shows similar protein distribution across tissues to an independent TPR Polyclonal Antibody (B).

Product Image Gallery

TPR Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of TPR in human cell line A-431 using TPR Polyclonal Antibody (Product # PA5-54048) shows localization to nuclear membrane.

TPR Antibody in Western Blot (WB) — Western blot analysis of TPR in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a TPR Polyclonal Antibody (Product # PA5-54048). Remaining relative intensity is presented.

TPR Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U2OS cells were transfected with, control siRNA, TPR siRNA probe #1, and TPR siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-TPR Polyclonal Antibody (Product # PA5-54048) used at a dilution of 0.4 µg/mL. {KD}

Product Details

PA5-54048

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human TPR

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2648855

Product Specific Information

Immunogen sequence: KMASVRQHLE ETTQKAESQL LECKASWEER ERMLKDEVSK CVCRCEDLEK QNRLLHDQIE KLSDKVVASV KEGVQGPLNV SLSEEGKSQE

Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 90%.

Target Information

Steroidogenic factor-1 (SF-1) regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. In the adult ovary, SF-1 localizes to theca/interstitial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Megator; NPC-associated intranuclear protein; nuclear pore complex-associated protein TPR; Nucleoprotein TPR; translocated promoter region (to activated MET oncogene); Translocated promoter region protein; tumor potentiating region

Gene Aliases: TPR

UniProt ID: (Human) P12270

Entrez Gene ID: (Human) 7175

Molecular Function: primary active transporter