Invitrogen

TPR Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (16) TPR antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

TPR Antibody in Immunofluorescence (IF) — Immunofluorescent staining of TPR in human cell line A-431 using TPR Polyclonal Antibody (Product # PA5-54048) shows localization to nuclear membrane.

TPR Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of TPR in human colon, kidney, lymph node and testis using TPR Polyclonal Antibody (Product # PA5-54048) (A) shows similar protein distribution across tissues to an independent TPR Polyclonal Antibody (B).

TPR Antibody in Western Blot (WB) — Western blot analysis of TPR in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a TPR Polyclonal Antibody (Product # PA5-54048). Remaining relative intensity is presented.

TPR Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U2OS cells were transfected with, control siRNA, TPR siRNA probe #1, and TPR siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-TPR Polyclonal Antibody (Product # PA5-54048) used at a dilution of 0.4 µg/ml. {KD}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Immunohistochemistry (IHC)

1:200-1:500

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human TPR

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2648855

Product Specific Information

Immunogen sequence: KMASVRQHLE ETTQKAESQL LECKASWEER ERMLKDEVSK CVCRCEDLEK QNRLLHDQIE KLSDKVVASV KEGVQGPLNV SLSEEGKSQE

Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 90%.

Target Information

Steroidogenic factor-1 (SF-1) regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. In the adult ovary, SF-1 localizes to theca/interstitial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Megator; NPC-associated intranuclear protein; nuclear pore complex-associated protein TPR; Nucleoprotein TPR; translocated promoter region (to activated MET oncogene); Translocated promoter region protein; tumor potentiating region

Gene Aliases: TPR

UniProt ID: (Human) P12270

Entrez Gene ID: (Human) 7175

Function(s)