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Invitrogen
TRAIL-R2 (DR5) Polyclonal Antibody
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FIGURE: 1 / 9
TRAIL-R2 (DR5) Antibody (PA1-957) in ICC/IF
Immunofluorescence analysis of TRAIL-R2 was performed using HCT 116 cells and HCT 116 treated with Thapsigargin (1 µM, 36 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TRAIL-R2 Rabbit Polyclonal Antibody (Product # PA1-957) at 2 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled wi... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-957
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C V(255) P E Q E M E V Q E P A E P T G(270)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-957 detects death receptor 5 (DR5) from human and rat tissues and cells.
PA1-957 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~58 kDa protein representing DR5 from HEK293 cell lysate.
PA1-957 immunizing peptide corresponds to amino acid residues 255-270 of rat DR5. PA1-957 immunizing peptide (Cat. # PEP-048) is available for use in neutralization and control experiments.
Target Information
DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by DR5. Two transcript variants encoding different isoforms of DR5 and one non-coding transcript have been found. Overexpression of DR5 induces apoptosis and activates NF-kappa-B. DR5 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. Diseases associated with DR5 dysfunction include squamous cell carcinoma, and diffuse infiltrative lymphocytosis syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD262; Death receptor 5; dr-5; KILLER/DR5 TRAIL death-inducing receptor; MK; sCD262; soluble CD262; TNF-related apoptosis-inducing ligand receptor 2; TRAIL receptor 2; Tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b
Gene Aliases: DR5; KILLER; Ly98; MK; TNFRSF10B; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; UNQ160/PRO186; ZTNFR9
UniProt ID: (Mouse) Q9QZM4
Entrez Gene ID: (Mouse) 21933, (Rat) 364420
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
signal transduction
extrinsic apoptotic signaling pathway via death domain receptors
TRAIL-activated apoptotic signaling pathway
regulation of apoptotic process
extrinsic apoptotic signaling pathway
male gonad development
response to organic substance
response to organic cyclic compound
cellular response to mechanical stimulus
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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