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Invitrogen
TRIB2 Polyclonal Antibody
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FIGURE: 1 / 2
TRIB2 Antibody (PA5-100925) in ICC/IF
Immunocytochemistry analysis of TRIB2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with TRIB2 polyclonal antibody (Product # PA5-100925) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100925
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TRIB2(Accession Q92519), corresponding to amino acid residues Y14-Y64.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TRIB2.
Target Information
This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: TRB-2; TRIB2; TRIB2 (HGNC:30809); Tribbles homolog 2
Gene Aliases: AW319517; C5FW; GS3955; TRB-2; TRB2; TRIB2
UniProt ID: (Human) Q92519, (Mouse) Q8K4K3
Entrez Gene ID: (Human) 28951, (Mouse) 217410
nucleotide binding
protein kinase activity
protein kinase inhibitor activity
transcription factor binding
mitogen-activated protein kinase kinase binding
ubiquitin protein ligase binding
ubiquitin-protein transferase regulator activity
ATP binding
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein phosphorylation
negative regulation of protein kinase activity
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of MAP kinase activity
negative regulation of interleukin-10 biosynthetic process
negative regulation of fat cell differentiation
negative regulation of sequence-specific DNA binding transcription factor activity
regulation of catalytic activity
positive regulation of ubiquitin-protein transferase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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