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TRIB3 Antibody (PA5-29887) in ICC/IF
Immunofluorescence analysis of Tribbles homolog 3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TRIB3 Polyclonal Antibody (Product # PA5-29887) at 1:300 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (P... View More
Product Details
PA5-29887
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 174 and 358 of Human TRIB3
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547361
Product Specific Information
Recommended positive controls: HepG2, HUVEC, HUVEC treat with Sphingosine 1-phosphate.
Predicted reactivity: Bovine (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
A novel inhibitor of cell division, Tribbles, blocks mitosis at a critical point in Drosophila morphogenesis. Recently, mammalian homologues of Tribbles (Trbs) have been identified that control mitogen-activated protein kinase (MAPK) activity. TRIB3 also known as TRB-3, SINK, and SKIP3 is a member of Trbs family. Overexpression of this protein inhibits NF-kB-dependent transcription induced by tumor necrosis factor (TNF) stimulation or its downstream signaling proteins but does not inhibit NF-kB translocation to the nucleus and binding to DNA. TRIB3/SKIP3 is also overexpressed in multiple human tumors and is regulated by hypoxia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DJ1103G7.3; neuronal cell death inducible putative kinase; Neuronal cell death-inducible putative kinase; p65-interacting inhibitor of NF-kappa-B; p65-interacting inhibitor of NF-kappaB; SINK; TRB-3; Tribbles 3; Tribbles homolog 3
Gene Aliases: C20orf97; NIPK; SINK; SKIP3; TRB3; TRIB3
UniProt ID: (Human) Q96RU7
Entrez Gene ID: (Human) 57761
transcription corepressor activity
protein kinase inhibitor activity
protein binding
ATP binding
kinase activity
protein kinase binding
mitogen-activated protein kinase kinase binding
ubiquitin protein ligase binding
ubiquitin-protein transferase regulator activity
non-receptor serine/threonine protein kinase
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
protein phosphorylation
negative regulation of protein kinase activity
regulation of glucose transport
positive regulation of protein binding
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
cellular response to insulin stimulus
response to endoplasmic reticulum stress
regulation of MAP kinase activity
negative regulation of fat cell differentiation
negative regulation of fatty acid biosynthetic process
negative regulation of transcription, DNA-templated
positive regulation of ubiquitin-protein transferase activity
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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