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Invitrogen
TRIM21 Polyclonal Antibody
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FIGURE: 1 / 3
TRIM21 Antibody (PA5-106903) in ICC/IF
Immunocytochemistry analysis of TRIM21 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with TRIM21 polyclonal antibody (Product # PA5-106903) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106903
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TRIM21(Accession P19474), corresponding to amino acid residues P444-Y475.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TRIM21.
Target Information
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; 52kD Ro/SSA autoantigen; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; Ro(SS-A); Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SS-A; tripartite motif protein 21; tripartite motif-containing 21; Tripartite motif-containing protein 21
Gene Aliases: RNF81; Ro/SSA; RO52; SSA; SSA1; TRIM21
UniProt ID: (Human) P19474
Entrez Gene ID: (Human) 6737, (Rat) 308901, (Mouse) 20821
DNA binding
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
identical protein binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein polyubiquitination
protein monoubiquitination
cell cycle
positive regulation of autophagy
protein ubiquitination
protein destabilization
negative regulation of NF-kappaB transcription factor activity
regulation of type I interferon production
negative regulation of viral transcription
response to interferon-gamma
innate immune response
positive regulation of cell cycle
positive regulation of viral entry into host cell
positive regulation of sequence-specific DNA binding transcription factor activity
protein autoubiquitination
interferon-gamma-mediated signaling pathway
protein trimerization
negative regulation of protein deubiquitination
negative regulation of viral release from host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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