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Invitrogen
TRIM28 Polyclonal Antibody
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FIGURE: 1 / 4
TRIM28 Antibody (PA5-35079) in ICC/IF
Immunocytochemistry analysis of TRIM28 in HeLa cells. Samples were incubated with TRIM28 polyclonal antibody (Product # PA5-35079) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). TRI... View More
Product Details
PA5-35079
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Amount
180 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; tripartite motif; Tripartite motif-containing protein 28; unnamed protein product
Gene Aliases: KAP1; PPP1R157; RNF96; TF1B; TIF1B; TIF1beta; TRIM28
UniProt ID: (Human) Q13263
Entrez Gene ID: (Human) 10155
DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
transcription corepressor activity
RNA binding
protein kinase activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
SUMO transferase activity
ubiquitin protein ligase binding
Krueppel-associated box domain binding
metal ion binding
ubiquitin protein ligase activity
ubiquitin-like protein ligase activity
chromo shadow domain binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
epithelial to mesenchymal transition
DNA repair
chromatin organization
methylation-dependent chromatin silencing
embryo implantation
positive regulation of macromolecule metabolic process
protein sumoylation
positive regulation of protein import into nucleus
DNA methylation involved in embryo development
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation by host of viral release from host cell
innate immune response
positive regulation of DNA repair
negative regulation of single stranded viral RNA replication via double stranded DNA intermediate
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of nucleobase-containing compound metabolic process
convergent extension involved in axis elongation
embryonic placenta morphogenesis
genetic imprinting
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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