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TRIM32 Polyclonal Antibody
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FIGURE: 1 / 3
TRIM32 Antibody (OST00344W-100UL) in IHC (P)
IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 mL 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instruct... View More
Product Details
OST00344W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from aa region 160-210 of mouse TRIM32 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The peptide is homologous in rat and shares 95% identity with human sequence.
Specificity of this antibody: TRIM32.
Target Information
TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: bM468K2.2 (tripartite motif protein 32); E3 ubiquitin-protein ligase TRIM32; RING-type E3 ubiquitin transferase TRIM32; RP11-67K19.1; tripartite motif protein 32; Tripartite motif-containing protein 32; zinc finger protein 117
Gene Aliases: 1810045E12Rik; 3f3; BBS11; Trim32; Zfp117
UniProt ID: (Mouse) Q8CH72
Entrez Gene ID: (Mouse) 69807, (Rat) 313264
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
myosin binding
ribosomal S6-glutamic acid ligase activity
translation initiation factor binding
ubiquitin binding
protein self-association
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
ubiquitin protein ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
molecular_function
protein polyubiquitination
tissue homeostasis
actin ubiquitination
response to UV
protein ubiquitination
positive regulation of cell growth
positive regulation of cell migration
negative regulation of viral transcription
response to tumor necrosis factor
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of neuron differentiation
positive regulation of protein catabolic process
positive regulation of cell cycle
positive regulation of proteolysis
positive regulation of neurogenesis
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
axon development
negative regulation of keratinocyte apoptotic process
negative regulation of viral release from host cell
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
positive regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of interleukin-17-mediated signaling pathway
positive regulation of chemokine (C-C motif) ligand 20 production
positive regulation of cell motility
biological_process
fat cell differentiation
negative regulation of fibroblast proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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