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Invitrogen
TSLP Polyclonal Antibody
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FIGURE: 1 / 9
TSLP Antibody (PA5-20320) in IHC (P)
Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using TSLP Polyclonal Antibody (Product # PA5-20320) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. ... View More
Product Details
PA5-20320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 19 amino acid peptide from near the center of human TSLP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is A-20 cell lysate.
PA5-20320 can be used with blocking peptide PEP-0440.
Target Information
TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytokine; short-chain four-helical bundle cytokine; Thymic stroma-derived lymphopoietin; Thymic stromal lymphopoietin
Gene Aliases: TSLP
UniProt ID: (Human) Q969D9, (Mouse) Q9JIE6
Entrez Gene ID: (Human) 85480, (Mouse) 53603
positive regulation of cytokine-mediated signaling pathway
JAK-STAT cascade
positive regulation of cell proliferation
cytokine-mediated signaling pathway
positive regulation of chemokine production
positive regulation of interleukin-10 production
positive regulation of interleukin-13 production
positive regulation of interleukin-5 production
positive regulation of interleukin-6 production
positive regulation of mast cell activation
interleukin-7-mediated signaling pathway
negative regulation of apoptotic process
positive regulation of inflammatory response
defense response to Gram-negative bacterium
defense response to fungus
antimicrobial humoral immune response mediated by antimicrobial peptide
positive regulation of chemokine (C-C motif) ligand 1 production
positive regulation of granulocyte colony-stimulating factor production
positive regulation of STAT cascade
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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