|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 19 amino acid peptide from near the center of human TSLP receptor.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||5 µg/ml|
|Western Blot (WB)||0.5-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
Despite its predicted molecular weight, TSLP-R often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is human liver tissue lysate.
PA5-20378 can be used with blocking peptide PEP-0495.
Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. It is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Its receptor TSLP-R will bind TSLP in a low-affinity fashion in transfected cells; co-transfection with IL-7R-alpha cDNA results in high-affinity binding and a functional heteromeric complex. This heteromeric receptor requires stat5 for TSLP-mediated signal transduction and is inhibited by SOCS-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increased expression of thymic stromal lymphopoietin and its receptor in Kimura's disease.
PA5-20378 was used in immunohistochemistry - paraffin section to determine the expression of thymic stromal lymphopoietin and its receptor in Kimura's disease
|Sakitani E,Nonaka M,Shibata N,Furukawa T,Yoshihara T||ORL; journal for oto-rhino-laryngology and its related specialties (77:44)||2015|