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|Tested species reactivity||Many|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Reacts with residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29677 detects Tet Repressor from many samples. This antibody works in any species where the TetR system is being used to regulate gene ezpression in mammalian cells.
PA1-29677 has been successfully used in Western blot and ELISA applications. Not suitable for immunofluorescence.
The PA1-29677 immunogen is residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes.
Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.
The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.