|Tested species reactivity||Bacteria|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4501 detects Tet repressor in bacteria samples.
PA1-4501 has been successfully used in Western blot and ELISA procedures.
The PA1-4501 immunogen is Residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein.
Maintain at 2-8°C of 3 months or at -20°C for longer periods. Avoid repeated freeze/thaw cycles.
The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.