Invitrogen

UBE1L Recombinant Polyclonal Antibody (7HCLC)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (9) UBE1L antibodies

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Datasheet

Protocols

Questions & Answers

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UBE1L Antibody in Western Blot (WB) — Immunoblot with UBE1L antibody (Product # 712170). NK-92, HeLa, Jurkat, and A549 cells were either untreated or IFN-b-treated for 48 hours as indicated. 8220Transfected8221 (last lane) represents extract from HEK293T cells transfected with plasmids expressing 3X-FLAG-ISG15, UBE1L, UBCH8, and HERC5. Cells were lysed in 1% NP40 lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 1% NP-40) with PMSF, aprotinin, leupeptin, and 1mM DTT. 30 mg total protein per sample were loaded onto a 13% Tris-Glycine PAGE gel, transferred to nitrocellulose using a semi-dry transfer apparatus (0.1A for 1 hour 15 minutes), and blocked for 30 minutes at RT. The membrane was probed with anti-ISG15 (1:1000 in 1X TNET) for one hour at RT, washed with 1X TNET, and incubated with secondary antibody (1:15,000) for one hour at RT. Protein marker used is Thermo Page Ruler Plus (Product # 26620). Data courtesy of Caleb Swaim and Jon Huibregtse at University of Texas at Austin.

UBE1L Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in UBE1L knockout (KO) cell line compared to control cell line using ABfinity™ Anti-UBE1L Recombinant Rabbit Oligoclonal Antibody (Product # 712170). {KO}

Product Details

712170

Applications	Tested Dilution	Publications
Western Blot (WB)	1:250	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Expression System	EXPI293
Class	Recombinant Polyclonal
Type	Antibody
Clone	7HCLC
Immunogen	Protein corresponding to human UBE1L [aa880-aa1012] View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2784613

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Horse, Cat.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

UBE1L (ubiquitin-like modifier-activating enzyme 7) activates ubiquitin by first adenylating with ATP at its C-terminal glycine residue and then linking that residue to the side chain of a cysteine residue in E1. This activation results in an ubiquitin-E1 thioester and free AMP. UBE1L also catalyzes the ISGylation of influenza A virus NS1 protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: D8; MGC12713; UBA1, ubiquitin-activating enzyme E1 homolog B; UBA7, ubiquitin-activating enzyme E1; Ubiquitin-activating enzyme 7; Ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-related protein; ubiquitin-activating enzyme-2; ubiquitin-like modifier activating enzyme 7; Ubiquitin-like modifier-activating enzyme 7

Gene Aliases: D8; UBA1B; UBA7; UBE1L; UBE2

UniProt ID: (Human) P41226

Entrez Gene ID: (Human) 7318

Molecular Function: ubiquitin-protein ligase

Function(s)

cellular protein modification process protein ubiquitination modification-dependent protein catabolic process translesion synthesis ISG15-protein conjugation negative regulation of type I interferon production

Process(es)

ubiquitin activating enzyme activity ubiquitin-protein transferase activity protein binding ATP binding ISG15 activating enzyme activity

UBE1L Recombinant Polyclonal Antibody (7HCLC)

Product Details

712170

Western Blot (WB)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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