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Immunofluorescent analysis of UBE2A showing staining in the cytoplasm and nucleus of A375 cells. A375 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a UBE2A polyclonal antibody (Product # PA5-27186) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 102 of Human UBE2A|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27186 targets UBE2A in WB applications and shows reactivity with Human samples.
The PA5-27186 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 102 of Human UBE2A.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
E2 ubiquitin-conjugating enzyme A; HHR6A; HR6A; MRXS30; MRXSN; RAD6 homolog A; RAD6A; UBC2; ubiquitin carrier protein A; ubiquitin conjugating enzyme E2A; ubiquitin-conjugating enzyme E2 A; ubiquitin-conjugating enzyme E2A (RAD6 homolog); ubiquitin-protein ligase A
HHR6A; MRXS30; MRXSN; RAD6A; UBC2; UBE2A