Invitrogen

UCKL1 Polyclonal Antibody

View all (9) UCKL1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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UCKL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of UCKL1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with UCKL1 Polyclonal Antibody (Product # PA5-118336, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

UCKL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human UCKL1 in human stomach with UCKL1 Polyclonal Antibody (Product # PA5-118336, 1:100 dilution, formalin-fixed paraffin embedded sections).

UCKL1 Antibody in Western Blot (WB) — Western Blot using UCKL1 Polyclonal Antibody (Product # PA5-118336) at 1:500 dilution. Lane A: 293T Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa. (We are unsure of the identity of these extra bands).

UCKL1 Antibody in Immunoprecipitation (IP) — UCKL1 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with UCKL1 Polyclonal Antibody (Product # PA5-118336) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: UCKL1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa.

Product Details

PA5-118336

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Immunoprecipitation (IP)	1-5 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human UCKL1 fragment
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% ProClin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2902930

Target Information

Uridine kinase is a key enzyme in the pyrimidine salvage pathway of all mammalian cells. URKL1 (uridine kinase-like 1) is highly similar in structure and sequence to uridine kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: UCK1-LIKE; uridine-cytidine kinase 1-like 1; Uridine-cytidine kinase-like 1

Gene Aliases: F538; UCK1L; UCKL1; URKL1

UniProt ID: (Human) Q9NWZ5

Entrez Gene ID: (Human) 54963

Molecular Function: nucleotide kinase

Function(s)

pyrimidine nucleobase metabolic process viral process phosphorylation pyrimidine nucleoside salvage UMP salvage CTP salvage

Process(es)

uridine kinase activity protein binding ATP binding

UCKL1 Polyclonal Antibody

Product Details

PA5-118336

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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