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Invitrogen
UCP1 Polyclonal Antibody
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FIGURE: 1 / 54
UCP1 Antibody (PA1-24894) in WB
Western blot was performed using Anti-UCP1 Polyclonal Antibody (Product # PA1-24894) and a 33 kDa band corresponding to UCP1 was observed only in Mouse Brown Adipose and not Mouse White Adipose. Tissue extracts (30 µg lysate) of Mouse Brown Adipose (Lane 1) and Mouse White Adipose (Lane 2) were electrophoresed using NuPAGE™ 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the pri... View More
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.jpg?time=20220826?time=20220826 "UCP1 Antibody (PA1-24894) in IHC (P)")
Product Details
PA1-24894
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat,
Ferret,
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Non-human primate,
Rat,
Rhesus monkey
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to human UCP1 sequence (amino acids 145-159) with N-terminal cysteine added, conjugated to KLH.
Conjugate
Form
Liquid
Concentration
1.3 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-24894 detects UCP1 from human, mouse, and rat samples, and does not cross-react with UCP2 or UCP3. PA1-24894 is expected to cross react with bovine (93% conserved), canine (93% conserved), Macaque monkey (100% conserved), rabbit (93% conserved), and ovine (93% conserved) due to sequence homology.
By Western Blot this antibody detects a band of approximately 32 kDa.
Positive Control: Brown adipose tissue.
Target Information
Mitochondrial uncoupling proteins are members of the family of mitochondrial anion carrier proteins. UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:12517; Mitochondrial brown fat uncoupling protein 1; Solute carrier family 25 member 7; Thermogenin; UCP 1; uncoupling protein; uncoupling protein 1 (mitochondrial, proton carrier); uncoupling protein 1, mitochondrial; uncoupling protein, mitochondrial
Gene Aliases: AI385626; SLC25A7; UCP; UCP1; Ucpa; Uncp
UniProt ID: (Human) P25874, (Rat) P04633, (Mouse) P12242
Entrez Gene ID: (Human) 7350, (Rat) 24860, (Mouse) 22227
regulation of transcription from RNA polymerase II promoter
translation
mitochondrial transport
proton transport
brown fat cell differentiation
generation of precursor metabolites and energy
cellular response to hormone stimulus
positive regulation of multicellular organismal metabolic process
response to steroid hormone
transport
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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