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|Tested species reactivity||Bovine, Dog, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 145-159 of human UCP1, with an N-terminal added cysteine.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24894 detects UCP1 from human, mouse, and rat samples, and does not cross-react with UCP2 or UCP3. PA1-24894 is expected to cross react with bovine (93% conserved), canine (93% conserved), Macaque monkey (100% conserved), rabbit (93% conserved), and ovine (93% conserved) due to sequence homology.
PA1-24894 has been successfully used in Immunohistochemistry (P), and Western Blot procedures.
By Western Blot this antibody detects a band of approximately 32 kDa.
The PA1-24894 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 145-159 of human UCP1, with an N-terminal added cysteine.
Positive Control: Brown adipose tissue.
For continous use, store at 2-8°C for up to one month. For long term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. Storage in frost-free freezers is not recommended.
Mitochondrial oxidative phosphorylation makes possible ATP synthesis using the energy available from substrate oxidation at the respiratory chain. These processes are coupled through the proton electrochemical potential gradient generated during the transfer of electrons from the substrate to oxygen. The uncoupling proteins (UCPs) are mitochondrial inner membrane proteins that are considered to be transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They are capable of returning protons pumped by the respiratory chain to the mitochondrial matrix. Uncoupling proteins currently comprise UCP1, UCP2, UCP3, UCP4, and UCP5. UCP1 is a 32 kDa protein that is active as a proton channelforming dimer. It can bind purine nucleotides and is capable of being stimulated by fatty acids. Proton transport by UCP1 has been shown to depend on CoQ (ubiquinone) as an obligatory cofactor. UCP1 is exclusively expressed in BAT in rodents and in neonates where it is regulated by norepinephrine and thyroid hormones. Stimulated BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration. The liberated heat can serve several physiological functions, e.g. for body heating during emergence from hibernation or during cold exposure, for burning body fat and consequently for body weight regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes.
PA1-24894 was used in western blot to investigate apelin signaling in brown adipocyte tissue
|Than A,He HL,Chua SH,Xu D,Sun L,Leow MK,Chen P||The Journal of biological chemistry (290:14679)||2015|
MiR-27 orchestrates the transcriptional regulation of brown adipogenesis.
PA1-24894 was used in immunocytochemistry and western blot to study the central transcriptional role of miRNA-27 in brown adipose tissue adipogenesis following cold exposure
|Sun L,Trajkovski M||Metabolism: clinical and experimental (63:272)||2014|
mitochondrial brown fat uncoupling protein 1; mitochondrial uncoupling protein 1; mitochondrial, proton carrier; SLC25A7; solute carrier family 25 member 7; thermogenin; UCP; UCP 1; uncoupling protein 1 (mitochondrial, proton carrier); uncoupling protein 1 UCP1; uncoupling protein 1, mitochondrial; uncoupling protein, mitochondrial
AI385626; BOS_16298; SLC25A7; UCP; UCP-1; UCP1; Ucpa; Uncp