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UEV1A Polyclonal Antibody
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FIGURE: 1 / 1
UEV1A Antibody (600-401-FU0) in WB
Western Blot of UEV1A antibody in Jurkat cell lysates. Lane A: UEV1A antibody at 1 µg/mL. Lane B: UEV1A antibody at 2 µg/mL. Lane C: UEV1A antibody at 4 µg/mL. Load: 35 µg per lane. Primary antibody: UEV1A antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 30 kDa, 16 kDa for UEV1A. Other band(s): UEV1A splice variants and isoforms.
Product Details
600-401-FU0
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-UEV1A antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to 15 amino acids near the C-terminus of human UEV1A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Ubiquitin-conjugating enzyme E2 (UEV1) was initially discovered as a protein similar in sequence and structure to the E2 ubiquitin-conjugating enzymes but lacking their enzymatic activity (1). There are at least two variants and multiple isoforms of UEV1. In particular, UEV1A (Ubiquitin-conjugating enzyme E2 variant 1 isoform A) has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). UEV1A is part of a dimeric ubiquitin-conjugating enzyme complex also containing Ubc13 (ubiquitin-conjugating enzyme 13) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CROC-1; DNA-binding protein; TRAF6-regulated IKK activator 1 beta Uev1A; TRIKA1beta; ubiquitin-conjugating enzyme E2 variant; Ubiquitin-conjugating enzyme E2 variant 1; UEV-1
Gene Aliases: 0610011J09Rik; AI256840; CIR1; CROC-1; CROC1; D7Bwg1382e; P/OKcl.19; UBE2V; UBE2V1; UEV-1; UEV1; UEV1A
UniProt ID: (Human) Q13404, (Mouse) Q9CZY3
Entrez Gene ID: (Human) 7335, (Rat) 296390, (Mouse) 66589
protein polyubiquitination
regulation of DNA repair
postreplication repair
regulation of transcription, DNA-templated
cell differentiation
error-free postreplication DNA repair
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription, DNA-templated
protein K63-linked ubiquitination
positive regulation of protein K63-linked ubiquitination
positive regulation of intracellular signal transduction
biological_process
positive regulation of NF-kappaB transcription factor activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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