Proteintech

USP2 Polyclonal Antibody

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USP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 10392-1-AP (USP2 antibody) at dilution of 1:100 (under 25x lens).

USP2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed mouse embryo tissue using 10392-1-AP (USP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

USP2 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10392-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

10392-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunohistochemistry (IHC)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	USP2 Fusion Protein Ag0591 (11-241 aa encoded by BC002955) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is specific to the isoform 1 (USP2a) of USP2.

Immunogen sequence: YTESARYTD AHYAKSGYGA YTPSSYGANL AASLLEKEKL GFKPVPTSSF LTRPRTYGPS SLLDYDRGRP LLRPDITGGG KRAESQTRGT ERPLGSGLSG GSGFPYGVTN NCLSYLPINA YDQGVTLTQK LDSQSDLARD FSSLRTSDSY RIDPRNLGRS PMLARTRKEL CTLQGLYQTA SCPEYLVDYL ENYGRKGSAS QVPSQAPPSR VPEIISPTYR PIGRYTLWET GK (11-241 aa encoded by BC002955)

Target Information

Ubiquitin is a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

3,3'-Diindolylmethane suppresses high-fat diet-induced obesity through inhibiting adipogenesis of pre-adipocytes by targeting USP2 activity.

Molecular nutrition & food research

Yang H,Seo SG,Shin SH,Min S,Kang MJ,Yoo R,Kwon JY,Yue S,Kim KH,Cheng JX,Kim JR,Park JS,Kim JH,Park JHY,Lee HJ,Lee KW

Sun Oct 01 00:00:00 EDT 2017

CMP-Neu5Ac Hydroxylase Null Mice as a Model for Studying Metabolic Disorders Caused by the Evolutionary Loss of Neu5Gc in Humans.

BioMed research international

Kwon DN,Choi YJ,Cho SG,Park C,Seo HG,Song H,Kim JH

Mon Aug 29 00:00:00 EDT 2016

USP2 promotes cell migration and invasion in triple negative breast cancer cell lines.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Qu Q,Mao Y,Xiao G,Fei X,Wang J,Zhang Y,Liu J,Cheng G,Chen X,Wang J,Shen K

Wed Jul 01 00:00:00 EDT 2015

Ubiquitin-specific protease 2-69 in macrophages potentially modulates metainflammation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kitamura H,Kimura S,Shimamoto Y,Okabe J,Ito M,Miyamoto T,Naoe Y,Kikuguchi C,Meek B,Toda C,Okamoto S,Kanehira K,Hase K,Watarai H,Ishizuka M,El-Osta A,Ohara O,Miyoshi I

Sun Dec 01 00:00:00 EST 2013

Expression of USP2-69 in mesangial cells in vivo and in vitro.

Pathology international

Wang S,Wu H,Liu Y,Sun J,Zhao Z,Chen Q,Guo M,Ma D,Zhang Z

Mon Mar 01 00:00:00 EST 2010

Expression of USP2-69 in mesangial cells in vivo and in vitro.

Pathology international

Wang S,Wu H,Liu Y,Sun J,Zhao Z,Chen Q,Guo M,Ma D,Zhang Z

Mon Mar 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: 41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin carboxyl-terminal hydrolase 2; ubiquitin specific protease 12; ubiquitin specific protease 2; ubiquitin specific protease 9; Ubiquitin thioesterase 2; ubiquitin thiolesterase 2; Ubiquitin-specific-processing protease 2; Ubiquitin-specific-processing protease testis; UBP-t

Gene Aliases: B930035K21Rik; MNCb-0190; UBP-t; UBP41; Ubp69; USP2; USP9

UniProt ID: (Human) O75604, (Mouse) O88623, (Rat) Q5U349

Entrez Gene ID: (Human) 9099, (Mouse) 53376, (Rat) 115771

Molecular Function: cysteine protease