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Agrisera
V-ATPase holoenzyme Polyclonal Antibody
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Product Details
AS17-4158
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
V-ATPase complex from Avena sativa.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Avena sativa
Target Information
The V-ATPase holoenzyme is a conserved, rotary ATP-driven proton pump found primarily on membranes of intracellular organelles (including endosomes, lysosomes, Golgi-derived compartments, and secretory vesicles) and, in specialized cell types, can also be present at the plasma membrane, where it establishes and maintains acidic luminal/extracellular pH. It is a multisubunit complex assembled from two sectors: a peripheral, cytosolic V1 domain that contains the ATP catalytic sites and a membrane-embedded V0 domain that forms the proton-translocation pathway. The V1 sector comprises eight subunits (A-H), organized around an (A3B3) catalytic head with additional structural/regulatory subunits forming central and peripheral stalk elements that couple ATP hydrolysis to rotation. The V0 sector contains the integral membrane a subunit plus a proteolipid ring built from c-family subunits and additional accessory subunits (including d and e), with the a subunit providing hemichannel routes that allow protons to access and exit the rotating proteolipid ring. By coupling ATP hydrolysis in V1 to rotation of the central rotor and proteolipid ring in V0, V-ATPase actively transports protons from the cytosol into organelle lumens, enabling organelle acidification required for protein sorting and trafficking, receptor-mediated endocytosis, protease/zymogen activation, and generation of proton electrochemical gradients that drive secondary transport processes such as neurotransmitter loading into synaptic vesicles; V-ATPase activity is also regulated in vivo by reversible dissociation of the V1 and V0 sectors and changes in coupling efficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: V-ATPase; V-type proton ATPase; vacuolar ATP synthase; vacuolar proton pump; vacuolar-type H+-ATPase; VATPase; VHA; VHA-A
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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