Invitrogen

VCP Polyclonal Antibody

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VCP Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of VCP in SHG-44 cells using a VCP Polyclonal antibody (Product # PA5-85940) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

VCP Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of VCP in SW480 cells using a VCP Polyclonal antibody (Product # PA5-85940) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

VCP Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of VCP in HepG2 cells using a VCP Polyclonal antibody (Product # PA5-85940) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

VCP Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of VCP in Hela cells using a VCP Polyclonal antibody (Product # PA5-85940) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

VCP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of VCP of paraffin-embedded Mouse skeletal muscle tissue using a VCP Polyclonal antibody (Product #PA5-85940). Counter stained with hematoxylin.

VCP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of VCP of paraffin-embedded Human uterus tissue using a VCP Polyclonal antibody (Product #PA5-85940). Counter stained with hematoxylin.

VCP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of VCP of paraffin-embedded Mouse heart tissue using a VCP Polyclonal antibody (Product #PA5-85940). Counter stained with hematoxylin.

VCP Antibody in Western Blot (WB) — Western blot analysis of VCP in different lysates using a Polyclonal antibody (Product #PA5-85940) at a dilution of 1:2000. Positive control: Lane 1: L929, Lane 2: F9, Lane 3: A549, Lane 4: MCF-7, Lane 5: Hela, Lane 6: NIH/3T3, Lane 7: N2A, Lane 8: Jurkat, Lane 9: HepG2, Lane 10: A431, Lane 11: Mouse heart, Lane 12: Mouse brain, Lane 13: Mouse liver, Lane 14: Mouse kidney.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:200

-

Immunofluorescence (IF)

1:200

-

Immunohistochemistry (IHC)

1:200

-

Western Blot (WB)

1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse, Zebrafish

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within C-terminal residues of VCP

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 40% Glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2802741

Target Information

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 15S Mg(2+)-ATPase p97 subunit; AAA ATPase p97; epididymis luminal protein 220; epididymis secretory protein Li 70; homolog of yeast cdc48; Inv protein; or HEL-S-70; p97 atpase; Protein CDC48; TER ATPase; Transitional endoplasmic reticulum ATPase; Valosin-containing protein; VCP; yeast Cdc48p homolog

Gene Aliases: 3110001E05; ALS14; CDC48; CMT2Y; HEL-220; HEL-S-70; IBMPFD; IBMPFD1; p97; p97/VCP; si:ch211-113n10.2; TERA; VCP; wu:fd16d05; wu:fj63d11

UniProt ID: (Human) P55072, (Mouse) Q01853, (Zebrafish) Q7ZU99

Entrez Gene ID: (Human) 7415, (Mouse) 269523, (Zebrafish) 327197

DNA repair double-strand break repair ubiquitin-dependent protein catabolic process transport ER to Golgi vesicle-mediated transport activation of cysteine-type endopeptidase activity involved in apoptotic process cellular response to DNA damage stimulus protein ubiquitination protein N-linked glycosylation via asparagine viral genome replication translesion synthesis ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol positive regulation of protein complex assembly positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein hexamerization positive regulation of protein catabolic process protein homooligomerization aggresome assembly ER-associated misfolded protein catabolic process positive regulation of protein K63-linked deubiquitination positive regulation of Lys63-specific deubiquitinase activity autophagy cell cycle locomotory behavior macroautophagy endosome to lysosome transport via multivesicular body sorting pathway chordate embryonic development mitotic spindle disassembly autophagosome maturation regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process NADH metabolic process positive regulation of mitochondrial membrane potential endoplasmic reticulum unfolded protein response ERAD pathway regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process establishment of protein localization ATP metabolic process error-free translesion synthesis flavin adenine dinucleotide catabolic process regulation of aerobic respiration positive regulation of oxidative phosphorylation positive regulation of ubiquitin-specific protease activity positive regulation of ATP biosynthetic process

Process(es)

protein binding ATP binding lipid binding ATPase activity protein phosphatase binding protein domain specific binding polyubiquitin binding ubiquitin protein ligase binding deubiquitinase activator activity K48-linked polyubiquitin binding MHC class I protein binding identical protein binding ADP binding ubiquitin-like protein ligase binding poly(A) RNA binding BAT3 complex binding ubiquitin-specific protease binding nucleotide binding receptor binding hydrolase activity protein complex binding