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VCP Antibody (MA3-004) in ICC/IF
Immunofluorescence analysis of VCP was performed using HeLa cells. The cells were fixed and permeabilized with ice cold 100% Acetone for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with VCP Monoclonal Antibody (5) (Product # MA3-004) at 5 microgram/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A28175) for 45 minutes at room temperature (Panel a: green)... View More
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.jpg?time=20220826?time=20220826 "VCP Antibody (MA3-004) in WB")
.jpg?time=20220826?time=20220826 "VCP Antibody (MA3-004) in IP")
Product Details
MA3-004
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Yeast
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
5
Immunogen
Synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-004 detects VCP protein in human, mouse, and rat samples.
MA3-004 has successfully been used in Western blot immunoprecipitation, and immunohistochemical procedures. By Western blot, this antibody detects an ~97 kDa protein representing VCP from total lysate of cultured human B cells.
The MA3-004 immunogen is a synthetic peptide corresponding to residues C G(792) G S V Y T E D N D D D L Y G(806) of mouse VCP. This peptide (Cat. # PEP-239) is available for use in neutralization and control experiments.
Target Information
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 15S Mg(2+)-ATPase p97 subunit; AAA ATPase p97; epididymis luminal protein 220; epididymis secretory protein Li 70; homolog of yeast cdc48; Inv protein; or HEL-S-70; p97 atpase; TER ATPase; Transitional endoplasmic reticulum ATPase; Valosin-containing protein; VCP; yeast Cdc48p homolog
Gene Aliases: 3110001E05; ALS14; CDC48; CMT2Y; HEL-220; HEL-S-70; IBMPFD; IBMPFD1; p97; p97/VCP; TERA; VCP
UniProt ID: (Human) P55072, (Mouse) Q01853, (Rat) P46462
Entrez Gene ID: (Human) 7415, (Mouse) 269523, (Rat) 116643
protein binding
ATP binding
lipid binding
ATPase activity
protein phosphatase binding
protein domain specific binding
polyubiquitin binding
ubiquitin protein ligase binding
deubiquitinase activator activity
K48-linked polyubiquitin binding
MHC class I protein binding
identical protein binding
ADP binding
ubiquitin-like protein ligase binding
poly(A) RNA binding
BAT3 complex binding
ubiquitin-specific protease binding
nucleotide binding
receptor binding
hydrolase activity
protein complex binding
DNA repair
double-strand break repair
ubiquitin-dependent protein catabolic process
transport
ER to Golgi vesicle-mediated transport
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular response to DNA damage stimulus
protein ubiquitination
protein N-linked glycosylation via asparagine
viral genome replication
translesion synthesis
ER-associated ubiquitin-dependent protein catabolic process
retrograde protein transport, ER to cytosol
positive regulation of protein complex assembly
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein hexamerization
positive regulation of protein catabolic process
protein homooligomerization
aggresome assembly
ER-associated misfolded protein catabolic process
positive regulation of protein K63-linked deubiquitination
positive regulation of Lys63-specific deubiquitinase activity
NADH metabolic process
autophagy
positive regulation of mitochondrial membrane potential
endoplasmic reticulum unfolded protein response
ERAD pathway
regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
establishment of protein localization
ATP metabolic process
error-free translesion synthesis
flavin adenine dinucleotide catabolic process
autophagosome maturation
regulation of aerobic respiration
positive regulation of oxidative phosphorylation
positive regulation of ubiquitin-specific protease activity
positive regulation of ATP biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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