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VDAC/Porin Polyclonal Antibody
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FIGURE: 1 / 4
VDAC/Porin Antibody (600-101-HB2) in ICC/IF
Immunofluorescence of Goat Anti-VDAC/Porin Antibody. Cell Line: HeLa Cells. Fixation: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-VDAC1/Porin at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 5µg/mL for 1hr at RT. Nuclear counter stain: DAPI. Location: Mitochondrion, Mitochondrion Outer Membrane, Nucleus. A). DAPI. B). V... View More
Product Details
600-101-HB2
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
VDAC/Porin Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to N-terminal portion of Human VDAC1/Porin1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 including mouse, rat, bovine, pig, and rabbit sources based on 100% homology for the immunogen sequence. Such broad reactivity makes this antibody useful as an excellent loading control.
Target Information
Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. It was discovered in the mitochondrial outer membrane. It also is expressed in the plasma membrane. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apoptosis. For example, VDAC constitutes a major pathway by which metabolites such as ADP/ATP, succinate and citrate are exchanged between the cytosol and mitochondria. And VDAC1 in the plasma membrane establishes a novel level of apoptosis regulation putatively via its redox activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1; ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 2; ATVDAC1; ATVDAC2; F2G14.210; HSR2; hVDAC1; K9I9.6; K9I9_6; MGC111064; Mitochondrial Porin; mVDAC1; mVDAC5; Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; PORIN-31-HL; rVDAC1; sperm binding protein 1a; T22N4.9; T22N4_9; unnamed protein product; VD; VDAC-1; VDAC-5; VDAC1; VDAC2; Voltage-dependent anion-selective channel protein 1; Voltage-dependent anion-selective channel protein 5
Gene Aliases: AL033343; PORIN; VDAC; VDAC-1; VDAC1; Vdac5
UniProt ID: (Human) P21796, (Mouse) Q60932, (Rat) Q9Z2L0
Entrez Gene ID: (Human) 7416, (Mouse) 22333, (Rat) 83529
nucleotide binding
voltage-gated ion channel activity
protein binding
ATP binding
oxysterol binding
lipid binding
voltage-gated anion channel activity
porin activity
cholesterol binding
protein kinase binding
phosphatidylcholine binding
identical protein binding
ion channel binding
ceramide binding
anion channel activity
protein complex binding
voltage-gated ion channel
behavioral fear response
ion transport
anion transport
lipid transport
apoptotic process
chemical synaptic transmission
neuron-neuron synaptic transmission
learning
epithelial cell differentiation
calcium ion transmembrane import into mitochondrion
positive regulation of apoptotic process
negative regulation of apoptotic process
transmembrane transport
anion transmembrane transport
negative regulation of calcium import into the mitochondrion
regulation of mitophagy
regulation of mitophagy
positive regulation of type 2 mitophagy
mitochondrial transmembrane transport
negative regulation of reactive oxygen species metabolic process
transport
mitochondrial calcium ion transport
regulation of anion transport
regulation of anion transmembrane transport
ion transmembrane transport
regulation of ion transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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