Invitrogen

VMAT2 Polyclonal Antibody

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VMAT2 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of VMAT2 was performed on human H1 huES teratoma tissue. To expose target proteins, antigen retrieval was performed using a pressure cooker in 10mM citric acid (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 2.5% hydrogen peroxide. Tissue slide was successively blocked with Avidin D, biotin and protein blocking buffer. Tissues were probed with a VMAT2 polyclonal antibody (Product # PA5-22864) diluted 1:100 overnight at 4°C. After washing tissue slide was incubated with biotinylated anti-rabbit IgG at 37°C for 30 min. After washing tissue slide was incubated at 37°C for 30 min with an HRP kit to conjugate biotinylated anti-rabbit IgG with biotinylated HRP/Avidin. DAB staining for development was performed for 5 min and tissue imaged. Data courtesy of the Antibody Data Exchange Program.

Product Details

PA5-22864

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:250

Immunocytochemistry (ICC/IF)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Reacts with an internal sequence of the human, mouse and rat VMAT2 protein.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154073

Target Information

VMAT2 is involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. It is a requisite for vesicular amine storage prior to secretion via exocytosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A role for glia maturation factor dependent activation of mast cells and microglia in MPTP induced dopamine loss and behavioural deficits in mice.

Brain, behavior, and immunity

Selvakumar GP,Ahmed ME,Thangavel R,Kempuraj D,Dubova I,Raikwar SP,Zaheer S,Iyer SS,Zaheer A

PA5-22864 was used in Western Blotting to investigate the molecular mechanism mediating degeneration of nigrostriatal dopaminergic neurons in Parkinson''s disease using methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced nigrostriatal neurodegeneration and astro-glial activations in mice.

Wed Jul 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: MGC120477; MGC120478; MGC26538; monoamine neurotransmitter transporter; Monoamine transporter; solute carrier family 18 (vesicular monoamine transporter), member 2; solute carrier family 18 (vesicular monoamine), member 2; Solute carrier family 18 A2 (vesicular monoamine transporter 2); Solute carrier family 18 member 2; solute carrier family 18, member 2; Synaptic vesicular amine transporter; VAT2; vesicle monoamine transporter type 2; vesicle monoamine/H+ antiporter; Vesicular amine transporter 2; vesicular monoamine transporter 2

Gene Aliases: 1110037L13Rik; 9330105E13; MNAT; SLC18A2; SVAT; SVMT; VAT2; VMAT-2; VMAT2

UniProt ID: (Human) Q05940, (Mouse) Q8BRU6, (Rat) Q01827

Entrez Gene ID: (Rabbit) 100351780, (Human) 6571, (Mouse) 214084, (Rat) 25549

Molecular Function: secondary carrier transporter