Invitrogen

VMAT2 Polyclonal Antibody

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VMAT2 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of VMAT2 was performed on human H1 huES teratoma tissue. To expose target proteins, antigen retrieval was performed using a pressure cooker in 10mM citric acid (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 2.5% hydrogen peroxide. Tissue slide was successively blocked with Avidin D, biotin and protein blocking buffer. Tissues were probed with a VMAT2 polyclonal antibody (Product # PA5-22864) diluted 1:100 overnight at 4°C. After washing tissue slide was incubated with biotinylated anti-rabbit IgG at 37°C for 30 min. After washing tissue slide was incubated at 37°C for 30 min with an HRP kit to conjugate biotinylated anti-rabbit IgG with biotinylated HRP/Avidin. DAB staining for development was performed for 5 min and tissue imaged. Data courtesy of the Antibody Data Exchange Program.

Product Details

PA5-22864

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:250	-

Product Specifications
Species Reactivity	Human, Mouse, Rabbit, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Reacts with an internal sequence of the human, mouse and rat VMAT2 protein.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11154073

Target Information

VMAT2 is involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. It is a requisite for vesicular amine storage prior to secretion via exocytosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC120477; MGC120478; MGC26538; monoamine neurotransmitter transporter; Monoamine transporter; solute carrier family 18 (vesicular monoamine transporter), member 2; solute carrier family 18 (vesicular monoamine), member 2; Solute carrier family 18 A2 (vesicular monoamine transporter 2); Solute carrier family 18 member 2; solute carrier family 18, member 2; Synaptic vesicular amine transporter; VAT2; vesicle monoamine transporter type 2; vesicle monoamine/H+ antiporter; Vesicular amine transporter 2; vesicular monoamine transporter 2

Gene Aliases: 1110037L13Rik; 9330105E13; MNAT; SLC18A2; SVAT; SVMT; VAT2; VMAT-2; VMAT2

UniProt ID: (Human) Q05940, (Mouse) Q8BRU6, (Rat) Q01827

Entrez Gene ID: (Rabbit) 100351780, (Human) 6571, (Mouse) 214084, (Rat) 25549

Molecular Function: secondary carrier transporter