Immunohistochemistry analysis of VMAT2 was performed on human H1 huES teratoma tissue. To expose target proteins, antigen retrieval was performed using a pressure cooker in 10mM citric acid (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 2.5% hydrogen peroxide. Tissue slide was successively blocked with Avidin D, biotin and protein blocking buffer. Tissues were probed with a VMAT2 polyclonal antibody (Product # PA5-22864) diluted 1:100 overnight at 4°C. After washing tissue slide was incubated with biotinylated anti-rabbit IgG at 37°C for 30 min. After washing tissue slide was incubated at 37°C for 30 min with an HRP kit to conjugate biotinylated anti-rabbit IgG with biotinylated HRP/Avidin. DAB staining for development was performed for 5 min and tissue imaged. Data courtesy of the Antibody Data Exchange Program.
|Tested species reactivity||Human, Mouse, Rabbit, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Reacts with an internal sequence of the human, mouse and rat VMAT2 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:250|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
VMAT2 is involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. It is a requisite for vesicular amine storage prior to secretion via exocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.