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Agrisera
VTG/Sole vitellogenin Polyclonal Antibody
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FIGURE: 1 / 1
VTG/Sole vitellogenin Antibody (AS06-127) in WB
STD: Protein standard (molecular weights of the proteins are indicated on the left) (1) plasma from male sole (load 0.11 µL of plasma), (2) plasma from vitellogenic female sole (load 0.11 µL of plasma), (3) plasma from estradiol-treated male sole (load 0.08 µL of plasma, (4) VTG precipitate (load 0.83 µL of precipitate), (5) purified VTG (load 2 µL of purified preparation, corresponding to around 0.14 µg an... View More
Product Details
AS06-127
Product Specifications
Species Reactivity
Fish
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Native vitellogenin purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis).
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Senegalese sole, Dicentrarchus labrax (sea bass), Sparus aurata (seabream)
Target Information
VTG encodes vitellogenin, a secreted yolk-precursor glycolipophosphoprotein that is synthesized predominantly in extra-ovarian tissue (classically liver in oviparous vertebrates) and released into the circulation for receptor-mediated uptake by growing oocytes during vitellogenesis, thereby supplying bulk nutrients required for embryonic development. At the protein level, vitellogenins are very large multidomain lipoproteins that enter the secretory pathway via an N-terminal signal peptide and share a conserved architecture comprising an N-terminal lipoprotein domain (vitellogenin_N/LPD_N), a central DUF1943 region, and a C-terminal von Willebrand factor type D-like domain; many taxa additionally produce characteristic yolk proteins through proteolytic processing of vitellogenin after ovarian uptake, yielding major yolk components such as lipovitellin heavy and light chains and, in some lineages, a serine-rich phosvitin region and other C-terminal fragments. VTG expression is strongly regulated by endocrine cues (notably estrogen-responsive control in liver), making VTG a core molecular effector linking reproductive hormonal signaling to deposition of lipid, phosphate, and other nutrient reserves into eggs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AtVGT1; T17B22.22; T17B22_22; vacuolar glucose transporter 1
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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