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VTI1B Polyclonal Antibody

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VTI1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — IHC-P on paraffin sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 mL 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Product Details

OSV00068W-100UL

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from the c-terminal region of human VTI1B conjugated to an immunogenic carrier protein was used as the antigen

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Specificity of this antibody: VTI1B.

Target Information

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence. Fischer von Mollard G., J. Biol. Chem. 273:2624-2630(1998). Li H.-C., Biochem. Biophys. Res. Commun. 247:70-74(1998).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

Analytical chemistry

Gauci S,Helbig AO,Slijper M,Krijgsveld J,Heck AJ,Mohammed S

Mon Jun 01 00:00:00 EDT 2009

Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.

Molecular cell

Daub H,Olsen JV,Bairlein M,Gnad F,Oppermann FS,Körner R,Greff Z,Kéri G,Stemmann O,Mann M

Fri Aug 08 00:00:00 EDT 2008

A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways.

The Journal of biological chemistry

Fischer von Mollard G,Stevens TH

Fri Jan 30 00:00:00 EST 1998

Bioinformatics

Protein Aliases: A novel SNARE; HGNC:17793; Soluble N-ethylmaleimide-sensitive factor-attachment protein receptor; vesicle transport through interaction with t-SNAREs 1B; vesicle transport through interaction with t-SNAREs 1B homolog; Vesicle transport through interaction with t-SNAREs homolog 1B; Vesicle transport v-SNARE protein Vti1-like 1; vesicle-associated soluble NSF attachment protein receptor; Vti1-rp1

Gene Aliases: AU015348; GES30; MVti1b; SNARE; v-SNARE; VTI1; VTI1-LIKE; Vti1-rp1; VTI1B; VTI1L; VTI1L1; VTI2

UniProt ID: (Human) Q9UEU0

Entrez Gene ID: (Human) 10490, (Rat) 100359512, (Mouse) 53612

Molecular Function: SNARE protein