Western blot analysis of mouse kidney tissue lysate using PA5-19842, Vinculin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19842 staining of EBS E14 TG2A Day 3 whole cell lysate at a dilution of 1 ug/ml (Lane 2). PA5-19842 staining of HepG2 whole cell lysate at a dilution of 1 ug/ml (lane 3). Lane 4: Vinculin antibody at 1 ug/ml + human skeletal muscle tissue lysate at 10ug Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse, Sheep, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1100 to the C-terminus of Human Vinculin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with horse, chicken, cow, pig, chimpanzee, zebrafish, rhesus monkey and zebra finch based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Patient-derived bladder cancer xenografts in the preclinical development of novel targeted therapies.
PA5-19842 was used in western blot to develop and characterize patient-derived primary xenografts to a model of muscle invasive bladder cancer.
|Jäger W,Xue H,Hayashi T,Janssen C,Awrey S,Wyatt AW,Anderson S,Moskalev I,Haegert A,Alshalalfa M,Erho N,Davicioni E,Fazli L,Li E,Collins C,Wang Y,Black PC||Oncotarget (6:21522)||2015|