CSI 004-18-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 004-18-02 cross-reacts with rabbit and horse vitronectin, no reactivity with human or sheep.
CSI 004-18-02 has been successfully used in ELISA and Immunohistochemistry techniques. CSI 004-18-02 is suitable in ELISA and immunostaining of frozen PLP-fixed sections of bovine tissues.
The antibody inhibits integrin-mediated cell adhesion to bovine vitronectin. It can be used to probe vitronectin conformation.
CSI 004-18-02 reacts weakly in ELISA with vitronectin coated directly onto the microtiter plate.
The CSI 004-18-02 immunogen is lysed bovine corneal endothelial cells and extracellular matrix.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.