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Invitrogen
Vitronectin Monoclonal Antibody (A27)
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FIGURE: 1 / 1
Vitronectin Antibody (CSI 004-27-02) in WB
Scientific reports 2021 -
Figure 4 Quantitative analysis of proteins in culture medium adsorbed by HA/CHA. ( a ) Western blots of Fibronectin, Vitronectin, and BSA remaining in culture medium after a 2 h incubation period in the presence of HA/CHA-covered monoliths with four HA/CHA microstructures. ( b ) Average normalized % adsorption of Fibronectin (blue), Vitronectin (red), and BSA (green) on HA/CHA-covered monoliths with four HA... View More
Product Details
CSI 004-27-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Sheep
Published species
Bovine,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
A27
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix.
Conjugate
Form
Liquid
Concentration
1.08 mg/mL
Amount
432 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
CSI 004-27-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 004-27-02 cross-reacts with sheep vitronectin, no reactivity with human or horse.
CSI 004-27-02 has been successfully used in ELISA, Western blotting and Immunohistochemistry techniques. CSI 004-27-02 is suitable for ELISA, immunoblotting and immunostaining of frozen PLP-fixed sections of bovine tissues. The antibody can be used as an affinity purification reagent of vitronectin from bovine plasma or serum and to quantitatively deplete plasma or serum of vitronectin. It can also be used to probe vitronectin conformation.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: vitronectin (serum spreading factor, somatomedin B, complement S-protein)
Entrez Gene ID: (Sheep) 101119231, (Bovine) 507525
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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