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Invitrogen
WIPI2 Polyclonal Antibody
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FIGURE: 1 / 9
WIPI2 Antibody (PA5-78465) in ICC/IF
Immunofluorescence analysis of WIPI2 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with WIPI2 Polyclonal Antibody (Product # PA5-78465) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a... View More
Product Details
PA5-78465
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human WIPI2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.57 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: K562, THP-1, Jurkat
Predicted Reactivity: Mouse (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: WD repeat domain phosphoinositide-interacting protein 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI-2; WIPI49-like protein 2
Gene Aliases: ATG18B; Atg21; CGI-50; WIPI-2; WIPI2
UniProt ID: (Human) Q9Y4P8, (Rat) Q6AY57
Entrez Gene ID: (Human) 26100, (Rat) 288498
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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